PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims: The presence of multiple Bacillus anthracis strains in different victims

Paul J. Jackson, Martin E. Hugh-Jones, Debra M. Adair, Gertrude Green, Karen K. Hill, Cheryl R. Kuske, Lev M. Grinberg, Faina A. Abramova, Paul S Keim

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

An outbreak of human anthrax occurred in Sverdlovsk, Union of Soviet Socialists Republic (now Ekaterinburg, Russia) in April 1979. Officials attributed this to consumption of contaminated meat, but Western governments believed it resulted from inhalation of spores accidentally released from a nearby military research facility. Tissue samples from 11 victims were obtained and methods of efficiently extracting high-quality total DNA from these samples were developed. Extracted DNA was analyzed by using PCR to determine whether it contained Bacillus anthracis-specific sequences. Double PCR using 'nested primers' increased sensitivity of the assay significantly. Tissue samples from 11 persons who died during the epidemic were examined. Results demonstrated that the entire complement of B. anthracis toxin and capsular antigen genes required for pathogenicity were present in tissues from each of these victims. Tissue from a vaccination site contained primarily nucleic acids from a live vaccine, although traces of genes from the infecting organisms were also present. PCR analysis using primers that detect the vrrA gene variable region on the B. anthracis chromosome demonstrated that at least four of the five known strain categories defined by this region were present in the tissue samples. Only one category is found in a single B. anthracis strain.

Original languageEnglish (US)
Pages (from-to)1224-1229
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number3
DOIs
StatePublished - Feb 3 1998

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Bacillus anthracis
Anthrax
Polymerase Chain Reaction
Military Facilities
Genes
USSR
Russia
DNA
Spores
Meat
Nucleic Acids
Inhalation
Disease Outbreaks
Virulence
Vaccination
Vaccines
Chromosomes
Antigens
Research

Keywords

  • Variable number tandem repeats
  • VrrA gene

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims : The presence of multiple Bacillus anthracis strains in different victims. / Jackson, Paul J.; Hugh-Jones, Martin E.; Adair, Debra M.; Green, Gertrude; Hill, Karen K.; Kuske, Cheryl R.; Grinberg, Lev M.; Abramova, Faina A.; Keim, Paul S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 3, 03.02.1998, p. 1224-1229.

Research output: Contribution to journalArticle

Jackson, Paul J. ; Hugh-Jones, Martin E. ; Adair, Debra M. ; Green, Gertrude ; Hill, Karen K. ; Kuske, Cheryl R. ; Grinberg, Lev M. ; Abramova, Faina A. ; Keim, Paul S. / PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims : The presence of multiple Bacillus anthracis strains in different victims. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 3. pp. 1224-1229.
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