Abstract
Arbuscular mycorrhizal (AM) fungi are widespread root symbionts that often improve the fitness of their plant hosts. We tested whether local adaptation in mycorrhizal symbioses would shape the community structure of these root symbionts in a way that maximizes their symbiotic functioning. We grew a native prairie grass (Andropogon gerardii) with all possible combinations of soils and AM fungal inocula from three different prairies that varied in soil characteristics and disturbance history (two native prairie remnants and one recently restored). We identified the AM fungi colonizing A. gerardii roots using PCR amplification and cloning of the small subunit rRNA gene. We observed 13 operational taxonomic units (OTUs) belonging to six genera in three families. Taxonomic richness was higher in the restored than the native prairies with one member of the Gigaspora dominating the roots of plants grown with inocula from native prairies. Inoculum source and the soil environment influenced the composition of AM fungi that colonized plant roots. Correspondingly, host plants and AM fungi responded significantly to the soil-inoculum combinations such that home fungi often had the highest fitness and provided the greatest benefit to A. gerardii. Similar patterns were observed within the soil-inoculum combinations originating from two native prairies, where five sequence types of a single Gigaspora OTU were virtually the only root colonizers. Our results indicate that indigenous assemblages of AM fungi were adapted to the local soil environment and that this process occurred both at a community scale and at the scale of fungal sequence types within a dominant OTU.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2573-2587 |
| Number of pages | 15 |
| Journal | Molecular Ecology |
| Volume | 22 |
| Issue number | 9 |
| DOIs | |
| State | Published - May 2013 |
Fingerprint
Keywords
- arbuscular mycorrhizas
- Gigaspora
- local adaptation
- prairies
- reciprocal inoculation
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
Cite this
Patterns of diversity and adaptation in Glomeromycota from three prairie grasslands. / Ji, Baoming; Gehring, Catherine A; Wilson, Gail W T; Miller, R. M.; Flores-Rentería, Lluvia; Johnson, Nancy.
In: Molecular Ecology, Vol. 22, No. 9, 05.2013, p. 2573-2587.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Patterns of diversity and adaptation in Glomeromycota from three prairie grasslands
AU - Ji, Baoming
AU - Gehring, Catherine A
AU - Wilson, Gail W T
AU - Miller, R. M.
AU - Flores-Rentería, Lluvia
AU - Johnson, Nancy
PY - 2013/5
Y1 - 2013/5
N2 - Arbuscular mycorrhizal (AM) fungi are widespread root symbionts that often improve the fitness of their plant hosts. We tested whether local adaptation in mycorrhizal symbioses would shape the community structure of these root symbionts in a way that maximizes their symbiotic functioning. We grew a native prairie grass (Andropogon gerardii) with all possible combinations of soils and AM fungal inocula from three different prairies that varied in soil characteristics and disturbance history (two native prairie remnants and one recently restored). We identified the AM fungi colonizing A. gerardii roots using PCR amplification and cloning of the small subunit rRNA gene. We observed 13 operational taxonomic units (OTUs) belonging to six genera in three families. Taxonomic richness was higher in the restored than the native prairies with one member of the Gigaspora dominating the roots of plants grown with inocula from native prairies. Inoculum source and the soil environment influenced the composition of AM fungi that colonized plant roots. Correspondingly, host plants and AM fungi responded significantly to the soil-inoculum combinations such that home fungi often had the highest fitness and provided the greatest benefit to A. gerardii. Similar patterns were observed within the soil-inoculum combinations originating from two native prairies, where five sequence types of a single Gigaspora OTU were virtually the only root colonizers. Our results indicate that indigenous assemblages of AM fungi were adapted to the local soil environment and that this process occurred both at a community scale and at the scale of fungal sequence types within a dominant OTU.
AB - Arbuscular mycorrhizal (AM) fungi are widespread root symbionts that often improve the fitness of their plant hosts. We tested whether local adaptation in mycorrhizal symbioses would shape the community structure of these root symbionts in a way that maximizes their symbiotic functioning. We grew a native prairie grass (Andropogon gerardii) with all possible combinations of soils and AM fungal inocula from three different prairies that varied in soil characteristics and disturbance history (two native prairie remnants and one recently restored). We identified the AM fungi colonizing A. gerardii roots using PCR amplification and cloning of the small subunit rRNA gene. We observed 13 operational taxonomic units (OTUs) belonging to six genera in three families. Taxonomic richness was higher in the restored than the native prairies with one member of the Gigaspora dominating the roots of plants grown with inocula from native prairies. Inoculum source and the soil environment influenced the composition of AM fungi that colonized plant roots. Correspondingly, host plants and AM fungi responded significantly to the soil-inoculum combinations such that home fungi often had the highest fitness and provided the greatest benefit to A. gerardii. Similar patterns were observed within the soil-inoculum combinations originating from two native prairies, where five sequence types of a single Gigaspora OTU were virtually the only root colonizers. Our results indicate that indigenous assemblages of AM fungi were adapted to the local soil environment and that this process occurred both at a community scale and at the scale of fungal sequence types within a dominant OTU.
KW - arbuscular mycorrhizas
KW - Gigaspora
KW - local adaptation
KW - prairies
KW - reciprocal inoculation
UR - http://www.scopus.com/inward/record.url?scp=84876676195&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876676195&partnerID=8YFLogxK
U2 - 10.1111/mec.12268
DO - 10.1111/mec.12268
M3 - Article
C2 - 23458035
AN - SCOPUS:84876676195
VL - 22
SP - 2573
EP - 2587
JO - Molecular Ecology
JF - Molecular Ecology
SN - 0962-1083
IS - 9
ER -