Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis

Cliff S. Han, Gary Xie, Jean F. Challacombe, Michael R. Altherr, Smriti S. Bhotika, David Bruce, Connie S. Campbell, Mary L. Campbell, Jin Chen, Olga Chertkov, Cathy Cleland, Mira Dimitrijevic, Norman A. Doggett, John J. Fawcett, Tijana Glavina, Lynne A. Goodwin, Karen K. Hill, Penny Hitchcock, Paul J. Jackson, Paul S Keim & 26 others Avinash Ramesh Kewalramani, Jon Longmire, Susan Lucas, Stephanie Malfatti, Kim McMurry, Linda J. Meincke, Monica Misra, Bernice L. Moseman, Mark Mundt, A. Christine Munk, Richard T. Okinaka, B. Parson-Quintana, Lee Philip Reilly, Paul Richardson, Donna L. Robinson, Eddy Rubin, Elizabeth Saunders, Roxanne Tapia, Judith G. Tesmer, Nina Thayer, Linda S. Thompson, Hope Tice, Lawrence O. Ticknor, Patti L. Wills, Thomas S. Brettin, Paul Gilna

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of A. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27 and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.

Original languageEnglish (US)
Pages (from-to)3382-3390
Number of pages9
JournalJournal of Bacteriology
Volume188
Issue number9
DOIs
StatePublished - May 2006

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Bacillus anthracis
Bacillus thuringiensis
Bacillus cereus
Sequence Analysis
Genome
Namibia
Equidae
Spores
Mental Competency
Virulence
Bacteria

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Han, C. S., Xie, G., Challacombe, J. F., Altherr, M. R., Bhotika, S. S., Bruce, D., ... Gilna, P. (2006). Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis. Journal of Bacteriology, 188(9), 3382-3390. https://doi.org/10.1128/JB.188.9.3382-3390.2006

Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis. / Han, Cliff S.; Xie, Gary; Challacombe, Jean F.; Altherr, Michael R.; Bhotika, Smriti S.; Bruce, David; Campbell, Connie S.; Campbell, Mary L.; Chen, Jin; Chertkov, Olga; Cleland, Cathy; Dimitrijevic, Mira; Doggett, Norman A.; Fawcett, John J.; Glavina, Tijana; Goodwin, Lynne A.; Hill, Karen K.; Hitchcock, Penny; Jackson, Paul J.; Keim, Paul S; Kewalramani, Avinash Ramesh; Longmire, Jon; Lucas, Susan; Malfatti, Stephanie; McMurry, Kim; Meincke, Linda J.; Misra, Monica; Moseman, Bernice L.; Mundt, Mark; Munk, A. Christine; Okinaka, Richard T.; Parson-Quintana, B.; Reilly, Lee Philip; Richardson, Paul; Robinson, Donna L.; Rubin, Eddy; Saunders, Elizabeth; Tapia, Roxanne; Tesmer, Judith G.; Thayer, Nina; Thompson, Linda S.; Tice, Hope; Ticknor, Lawrence O.; Wills, Patti L.; Brettin, Thomas S.; Gilna, Paul.

In: Journal of Bacteriology, Vol. 188, No. 9, 05.2006, p. 3382-3390.

Research output: Contribution to journalArticle

Han, CS, Xie, G, Challacombe, JF, Altherr, MR, Bhotika, SS, Bruce, D, Campbell, CS, Campbell, ML, Chen, J, Chertkov, O, Cleland, C, Dimitrijevic, M, Doggett, NA, Fawcett, JJ, Glavina, T, Goodwin, LA, Hill, KK, Hitchcock, P, Jackson, PJ, Keim, PS, Kewalramani, AR, Longmire, J, Lucas, S, Malfatti, S, McMurry, K, Meincke, LJ, Misra, M, Moseman, BL, Mundt, M, Munk, AC, Okinaka, RT, Parson-Quintana, B, Reilly, LP, Richardson, P, Robinson, DL, Rubin, E, Saunders, E, Tapia, R, Tesmer, JG, Thayer, N, Thompson, LS, Tice, H, Ticknor, LO, Wills, PL, Brettin, TS & Gilna, P 2006, 'Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis', Journal of Bacteriology, vol. 188, no. 9, pp. 3382-3390. https://doi.org/10.1128/JB.188.9.3382-3390.2006
Han, Cliff S. ; Xie, Gary ; Challacombe, Jean F. ; Altherr, Michael R. ; Bhotika, Smriti S. ; Bruce, David ; Campbell, Connie S. ; Campbell, Mary L. ; Chen, Jin ; Chertkov, Olga ; Cleland, Cathy ; Dimitrijevic, Mira ; Doggett, Norman A. ; Fawcett, John J. ; Glavina, Tijana ; Goodwin, Lynne A. ; Hill, Karen K. ; Hitchcock, Penny ; Jackson, Paul J. ; Keim, Paul S ; Kewalramani, Avinash Ramesh ; Longmire, Jon ; Lucas, Susan ; Malfatti, Stephanie ; McMurry, Kim ; Meincke, Linda J. ; Misra, Monica ; Moseman, Bernice L. ; Mundt, Mark ; Munk, A. Christine ; Okinaka, Richard T. ; Parson-Quintana, B. ; Reilly, Lee Philip ; Richardson, Paul ; Robinson, Donna L. ; Rubin, Eddy ; Saunders, Elizabeth ; Tapia, Roxanne ; Tesmer, Judith G. ; Thayer, Nina ; Thompson, Linda S. ; Tice, Hope ; Ticknor, Lawrence O. ; Wills, Patti L. ; Brettin, Thomas S. ; Gilna, Paul. / Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis. In: Journal of Bacteriology. 2006 ; Vol. 188, No. 9. pp. 3382-3390.
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abstract = "Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis are closely related gram-positive, spore-forming bacteria of the B. cereus sensu lato group. While independently derived strains of B. anthracis reveal conspicuous sequence homogeneity, environmental isolates of B. cereus and B. thuringiensis exhibit extensive genetic diversity. Here we report the sequencing and comparative analysis of the genomes of two members of the B. cereus group, B. thuringiensis 97-27 subsp. konkukian serotype H34, isolated from a necrotic human wound, and B. cereus E33L, which was isolated from a swab of a zebra carcass in Namibia. These two strains, when analyzed by amplified fragment length polymorphism within a collection of over 300 of A. cereus, B. thuringiensis, and B. anthracis isolates, appear closely related to B. anthracis. The B. cereus E33L isolate appears to be the nearest relative to B. anthracis identified thus far. Whole-genome sequencing of B. thuringiensis 97-27 and B. cereus E33L was undertaken to identify shared and unique genes among these isolates in comparison to the genomes of pathogenic strains B. anthracis Ames and B. cereus G9241 and nonpathogenic strains B. cereus ATCC 10987 and B. cereus ATCC 14579. Comparison of these genomes revealed differences in terms of virulence, metabolic competence, structural components, and regulatory mechanisms.",
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T1 - Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis

AU - Han, Cliff S.

AU - Xie, Gary

AU - Challacombe, Jean F.

AU - Altherr, Michael R.

AU - Bhotika, Smriti S.

AU - Bruce, David

AU - Campbell, Connie S.

AU - Campbell, Mary L.

AU - Chen, Jin

AU - Chertkov, Olga

AU - Cleland, Cathy

AU - Dimitrijevic, Mira

AU - Doggett, Norman A.

AU - Fawcett, John J.

AU - Glavina, Tijana

AU - Goodwin, Lynne A.

AU - Hill, Karen K.

AU - Hitchcock, Penny

AU - Jackson, Paul J.

AU - Keim, Paul S

AU - Kewalramani, Avinash Ramesh

AU - Longmire, Jon

AU - Lucas, Susan

AU - Malfatti, Stephanie

AU - McMurry, Kim

AU - Meincke, Linda J.

AU - Misra, Monica

AU - Moseman, Bernice L.

AU - Mundt, Mark

AU - Munk, A. Christine

AU - Okinaka, Richard T.

AU - Parson-Quintana, B.

AU - Reilly, Lee Philip

AU - Richardson, Paul

AU - Robinson, Donna L.

AU - Rubin, Eddy

AU - Saunders, Elizabeth

AU - Tapia, Roxanne

AU - Tesmer, Judith G.

AU - Thayer, Nina

AU - Thompson, Linda S.

AU - Tice, Hope

AU - Ticknor, Lawrence O.

AU - Wills, Patti L.

AU - Brettin, Thomas S.

AU - Gilna, Paul

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