Oxidative stress in older adults: Effects of physical fitness

Tinna Traustadottir, Sean S. Davies, Yali Su, Leena Choi, Holly M. Brown-Borg, L. Jackson Roberts, S. Mitchell Harman

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Acute exercise results in transient change in redox balance. High concentrations of reactive oxygen species (ROS) can lead to oxidative damage to macromolecules. However, moderate periodic increases in ROS, such as experienced with habitual exercise, may activate signal transduction pathways which stimulate increases in endogenous antioxidant systems. This study tested the hypothesis that physically fit older adults would have less oxidative stress than unfit age-matched controls, due to greater circulating concentrations of non-enzymatic antioxidants and greater capacity to upregulate antioxidant enzymes. We compared 37 fit (mean age 65.2±5 years) and 35 unfit (mean age 67.7± 4 years) men and women. Fitness status was classified by VO2 max and maximal leg power. Basal levels of oxidative stress were assessed by measuring urinary markers of nucleic acid damage and lipid peroxidation. Antioxidant status was assessed by measuring total antioxidant power and ratios of reduced to oxidized glutathione in plasma, at rest. The capacity to counteract an oxidative insult was assessed by measuring changes in plasma F2-isoprostanes in response to forearm ischemia-reperfusion. The fit individuals had significantly lower levels of urinary markers of oxidative damage (all P<0.05) and lower F2-isoprostane response to the oxidative challenge (P<0.05), but there were no group differences in antioxidant status. The lower levels of oxidative stress in the fit individuals were not mediated by known effects of exercise training such as adiposity, HDL concentrations, or small molecular weight antioxidants. These data suggest that reduced oxidative stress associated with physical fitness results from differences in activity of antioxidant enzymes.

Original languageEnglish (US)
Pages (from-to)969-982
Number of pages14
JournalAge
Volume34
Issue number4
DOIs
StatePublished - Aug 2012
Externally publishedYes

Fingerprint

Physical Fitness
Oxidative Stress
Antioxidants
F2-Isoprostanes
Exercise
Reactive Oxygen Species
Glutathione Disulfide
Adiposity
Enzymes
Forearm
Nucleic Acids
Lipid Peroxidation
Reperfusion
Oxidation-Reduction
Signal Transduction
Leg
Up-Regulation
Ischemia
Molecular Weight

Keywords

  • 8-Hydroxy-2'-deoxyguanosine
  • Exercise
  • F2-isoprostanes
  • Glutathione
  • Ischemia-reperfusion

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Traustadottir, T., Davies, S. S., Su, Y., Choi, L., Brown-Borg, H. M., Roberts, L. J., & Harman, S. M. (2012). Oxidative stress in older adults: Effects of physical fitness. Age, 34(4), 969-982. https://doi.org/10.1007/s11357-011-9277-6

Oxidative stress in older adults : Effects of physical fitness. / Traustadottir, Tinna; Davies, Sean S.; Su, Yali; Choi, Leena; Brown-Borg, Holly M.; Roberts, L. Jackson; Harman, S. Mitchell.

In: Age, Vol. 34, No. 4, 08.2012, p. 969-982.

Research output: Contribution to journalArticle

Traustadottir, T, Davies, SS, Su, Y, Choi, L, Brown-Borg, HM, Roberts, LJ & Harman, SM 2012, 'Oxidative stress in older adults: Effects of physical fitness', Age, vol. 34, no. 4, pp. 969-982. https://doi.org/10.1007/s11357-011-9277-6
Traustadottir T, Davies SS, Su Y, Choi L, Brown-Borg HM, Roberts LJ et al. Oxidative stress in older adults: Effects of physical fitness. Age. 2012 Aug;34(4):969-982. https://doi.org/10.1007/s11357-011-9277-6
Traustadottir, Tinna ; Davies, Sean S. ; Su, Yali ; Choi, Leena ; Brown-Borg, Holly M. ; Roberts, L. Jackson ; Harman, S. Mitchell. / Oxidative stress in older adults : Effects of physical fitness. In: Age. 2012 ; Vol. 34, No. 4. pp. 969-982.
@article{721404e6ea2c48e49994ab8f236da075,
title = "Oxidative stress in older adults: Effects of physical fitness",
abstract = "Acute exercise results in transient change in redox balance. High concentrations of reactive oxygen species (ROS) can lead to oxidative damage to macromolecules. However, moderate periodic increases in ROS, such as experienced with habitual exercise, may activate signal transduction pathways which stimulate increases in endogenous antioxidant systems. This study tested the hypothesis that physically fit older adults would have less oxidative stress than unfit age-matched controls, due to greater circulating concentrations of non-enzymatic antioxidants and greater capacity to upregulate antioxidant enzymes. We compared 37 fit (mean age 65.2±5 years) and 35 unfit (mean age 67.7± 4 years) men and women. Fitness status was classified by VO2 max and maximal leg power. Basal levels of oxidative stress were assessed by measuring urinary markers of nucleic acid damage and lipid peroxidation. Antioxidant status was assessed by measuring total antioxidant power and ratios of reduced to oxidized glutathione in plasma, at rest. The capacity to counteract an oxidative insult was assessed by measuring changes in plasma F2-isoprostanes in response to forearm ischemia-reperfusion. The fit individuals had significantly lower levels of urinary markers of oxidative damage (all P<0.05) and lower F2-isoprostane response to the oxidative challenge (P<0.05), but there were no group differences in antioxidant status. The lower levels of oxidative stress in the fit individuals were not mediated by known effects of exercise training such as adiposity, HDL concentrations, or small molecular weight antioxidants. These data suggest that reduced oxidative stress associated with physical fitness results from differences in activity of antioxidant enzymes.",
keywords = "8-Hydroxy-2'-deoxyguanosine, Exercise, F2-isoprostanes, Glutathione, Ischemia-reperfusion",
author = "Tinna Traustadottir and Davies, {Sean S.} and Yali Su and Leena Choi and Brown-Borg, {Holly M.} and Roberts, {L. Jackson} and Harman, {S. Mitchell}",
year = "2012",
month = "8",
doi = "10.1007/s11357-011-9277-6",
language = "English (US)",
volume = "34",
pages = "969--982",
journal = "GeroScience",
issn = "2509-2715",
publisher = "Springer International Publishing AG",
number = "4",

}

TY - JOUR

T1 - Oxidative stress in older adults

T2 - Effects of physical fitness

AU - Traustadottir, Tinna

AU - Davies, Sean S.

AU - Su, Yali

AU - Choi, Leena

AU - Brown-Borg, Holly M.

AU - Roberts, L. Jackson

AU - Harman, S. Mitchell

PY - 2012/8

Y1 - 2012/8

N2 - Acute exercise results in transient change in redox balance. High concentrations of reactive oxygen species (ROS) can lead to oxidative damage to macromolecules. However, moderate periodic increases in ROS, such as experienced with habitual exercise, may activate signal transduction pathways which stimulate increases in endogenous antioxidant systems. This study tested the hypothesis that physically fit older adults would have less oxidative stress than unfit age-matched controls, due to greater circulating concentrations of non-enzymatic antioxidants and greater capacity to upregulate antioxidant enzymes. We compared 37 fit (mean age 65.2±5 years) and 35 unfit (mean age 67.7± 4 years) men and women. Fitness status was classified by VO2 max and maximal leg power. Basal levels of oxidative stress were assessed by measuring urinary markers of nucleic acid damage and lipid peroxidation. Antioxidant status was assessed by measuring total antioxidant power and ratios of reduced to oxidized glutathione in plasma, at rest. The capacity to counteract an oxidative insult was assessed by measuring changes in plasma F2-isoprostanes in response to forearm ischemia-reperfusion. The fit individuals had significantly lower levels of urinary markers of oxidative damage (all P<0.05) and lower F2-isoprostane response to the oxidative challenge (P<0.05), but there were no group differences in antioxidant status. The lower levels of oxidative stress in the fit individuals were not mediated by known effects of exercise training such as adiposity, HDL concentrations, or small molecular weight antioxidants. These data suggest that reduced oxidative stress associated with physical fitness results from differences in activity of antioxidant enzymes.

AB - Acute exercise results in transient change in redox balance. High concentrations of reactive oxygen species (ROS) can lead to oxidative damage to macromolecules. However, moderate periodic increases in ROS, such as experienced with habitual exercise, may activate signal transduction pathways which stimulate increases in endogenous antioxidant systems. This study tested the hypothesis that physically fit older adults would have less oxidative stress than unfit age-matched controls, due to greater circulating concentrations of non-enzymatic antioxidants and greater capacity to upregulate antioxidant enzymes. We compared 37 fit (mean age 65.2±5 years) and 35 unfit (mean age 67.7± 4 years) men and women. Fitness status was classified by VO2 max and maximal leg power. Basal levels of oxidative stress were assessed by measuring urinary markers of nucleic acid damage and lipid peroxidation. Antioxidant status was assessed by measuring total antioxidant power and ratios of reduced to oxidized glutathione in plasma, at rest. The capacity to counteract an oxidative insult was assessed by measuring changes in plasma F2-isoprostanes in response to forearm ischemia-reperfusion. The fit individuals had significantly lower levels of urinary markers of oxidative damage (all P<0.05) and lower F2-isoprostane response to the oxidative challenge (P<0.05), but there were no group differences in antioxidant status. The lower levels of oxidative stress in the fit individuals were not mediated by known effects of exercise training such as adiposity, HDL concentrations, or small molecular weight antioxidants. These data suggest that reduced oxidative stress associated with physical fitness results from differences in activity of antioxidant enzymes.

KW - 8-Hydroxy-2'-deoxyguanosine

KW - Exercise

KW - F2-isoprostanes

KW - Glutathione

KW - Ischemia-reperfusion

UR - http://www.scopus.com/inward/record.url?scp=84866482979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866482979&partnerID=8YFLogxK

U2 - 10.1007/s11357-011-9277-6

DO - 10.1007/s11357-011-9277-6

M3 - Article

C2 - 21671197

AN - SCOPUS:84866482979

VL - 34

SP - 969

EP - 982

JO - GeroScience

JF - GeroScience

SN - 2509-2715

IS - 4

ER -