Overexpression of translation elongation factor 1A affects the organization and function of the actin cytoskeleton in yeast

R. Munshi, K. A. Kandl, A. Carr-Schmid, J. L. Whitacre, Alison Adams, T. G. Kinzy

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The translation elongation factor 1 complex (eEF1) plays a central role in protein synthesis, delivering aminoacyl-tRNAs to the elongating ribosome. The eEF1A subunit, a classic G-protein, also performs roles aside from protein synthesis. The overexpression of either eEF1A or eEF1Bα, the catalytic subunit of the guanine nucleotide exchange factor, in Saccharomyces cerevisiae results in effects on cell growth. Here we demonstrate that overexpression of either factor does not affect the levels of the other subunit or the rate or accuracy of protein synthesis. Instead, the major effects in vivo appear to be at the level of cell morphology and budding. eEF1A overexpression results in dosage-dependent reduced budding and altered actin distribution and cellular morphology. In addition, the effects of excess eEF1A in actin mutant strains show synthetic growth defects, establishing a genetic connection between the two proteins. As the ability of eEF1A to bind and bundle actin is conserved in yeast, these results link the established ability of eEF1A to bind and bundle actin in vitro with nontranslational roles for the protein in vivo.

Original languageEnglish (US)
Pages (from-to)1425-1436
Number of pages12
JournalGenetics
Volume157
Issue number4
StatePublished - 2001
Externally publishedYes

Fingerprint

Peptide Elongation Factors
Actin Cytoskeleton
Yeasts
Actins
Proteins
Peptide Elongation Factor 1
Guanine Nucleotide Exchange Factors
Growth
Transfer RNA
Ribosomes
GTP-Binding Proteins
Saccharomyces cerevisiae
Catalytic Domain

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Munshi, R., Kandl, K. A., Carr-Schmid, A., Whitacre, J. L., Adams, A., & Kinzy, T. G. (2001). Overexpression of translation elongation factor 1A affects the organization and function of the actin cytoskeleton in yeast. Genetics, 157(4), 1425-1436.

Overexpression of translation elongation factor 1A affects the organization and function of the actin cytoskeleton in yeast. / Munshi, R.; Kandl, K. A.; Carr-Schmid, A.; Whitacre, J. L.; Adams, Alison; Kinzy, T. G.

In: Genetics, Vol. 157, No. 4, 2001, p. 1425-1436.

Research output: Contribution to journalArticle

Munshi, R, Kandl, KA, Carr-Schmid, A, Whitacre, JL, Adams, A & Kinzy, TG 2001, 'Overexpression of translation elongation factor 1A affects the organization and function of the actin cytoskeleton in yeast', Genetics, vol. 157, no. 4, pp. 1425-1436.
Munshi, R. ; Kandl, K. A. ; Carr-Schmid, A. ; Whitacre, J. L. ; Adams, Alison ; Kinzy, T. G. / Overexpression of translation elongation factor 1A affects the organization and function of the actin cytoskeleton in yeast. In: Genetics. 2001 ; Vol. 157, No. 4. pp. 1425-1436.
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