Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms

A. S. Roy, S. M. Gibbons, H. Schunck, S. Owens, James G Caporaso, M. Sperling, J. I. Nissimov, S. Romac, L. Bittner, M. Mühling, U. Riebesell, J. LaRoche, J. A. Gilbert

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Abstract

The impact of ocean acidification and carbonation on microbial community structure was assessed during a large-scale in situ costal pelagic mesocosm study, included as part of the EPOCA 2010 Arctic campaign. The mesocosm experiment included ambient conditions (fjord) and nine mesocosms with pCO2 levels ranging from ∼ 145 to ∼ 1420μatm. Samples for the present study were collected at ten time points (t - 1, t1, t5, t7, t12, t14, t18, t22, t26 to t28) in seven treatments (ambient fjord (∼ 145), 2 × ∼ 185, ∼270, ∼685, ∼ 820, ∼ 1050μatm) and were analysed for "small" and "large" size fraction microbial community composition using 16S rRNA (ribosomal ribonucleic acid) amplicon sequencing. This high-throughput sequencing analysis produced ∼ 20 000 000 16S rRNA V4 reads, which comprised 7000 OTUs. The main variables structuring these communities were sample origins (fjord or mesocosms) and the community size fraction (small or large size fraction). The community was significantly different between the unenclosed fjord water and enclosed mesocosms (both control and elevated CO2 treatments) after nutrients were added to the mesocosms, suggesting that the addition of nutrients is the primary driver of the change in mesocosm community structure. The relative importance of each structuring variable depended greatly on the time at which the community was sampled in relation to the phytoplankton bloom. The sampling strategy of separating the small and large size fraction was the second most important factor for community structure. When the small and large size fraction bacteria were analysed separately at different time points, the only taxon pCO2 was found to significantly affect were the Gammaproteobacteria after nutrient addition. Finally, pCO2 treatment was found to be significantly correlated (non-linear) with 15 rare taxa, most of which increased in abundance with higher CO2.

Original languageEnglish (US)
Pages (from-to)555-566
Number of pages12
JournalBiogeosciences
Volume10
Issue number1
DOIs
StatePublished - Jan 29 2013

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bacterial communities
fjord
community structure
mesocosm
microbial communities
nutrients
carbonation
RNA
Otus
gamma-Proteobacteria
nutrient
microbial community
algal blooms
sampling
Arctic region
bacteria
community composition
algal bloom
phytoplankton
ocean acidification

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Earth-Surface Processes

Cite this

Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms. / Roy, A. S.; Gibbons, S. M.; Schunck, H.; Owens, S.; Caporaso, James G; Sperling, M.; Nissimov, J. I.; Romac, S.; Bittner, L.; Mühling, M.; Riebesell, U.; LaRoche, J.; Gilbert, J. A.

In: Biogeosciences, Vol. 10, No. 1, 29.01.2013, p. 555-566.

Research output: Contribution to journalArticle

Roy, AS, Gibbons, SM, Schunck, H, Owens, S, Caporaso, JG, Sperling, M, Nissimov, JI, Romac, S, Bittner, L, Mühling, M, Riebesell, U, LaRoche, J & Gilbert, JA 2013, 'Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms', Biogeosciences, vol. 10, no. 1, pp. 555-566. https://doi.org/10.5194/bg-10-555-2013
Roy, A. S. ; Gibbons, S. M. ; Schunck, H. ; Owens, S. ; Caporaso, James G ; Sperling, M. ; Nissimov, J. I. ; Romac, S. ; Bittner, L. ; Mühling, M. ; Riebesell, U. ; LaRoche, J. ; Gilbert, J. A. / Ocean acidification shows negligible impacts on high-latitude bacterial community structure in coastal pelagic mesocosms. In: Biogeosciences. 2013 ; Vol. 10, No. 1. pp. 555-566.
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