Nucleotide sequence and chromosomal location of Cab11 and Cab12, the genes for the fourth polypeptide of the photosystem I light-harvesting antenna (LHCI)

Egbert Schwartz, Dingren Shen, Ruedi Aebersold, J. Mitchell McGrath, Eran Pichersky, Beverley R. Green

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Tryptic peptide sequences from the 22 kDa polypeptide of tomato LHCI were used to construct a probe for gene cloning. The two genes cloned, cab11 and cab12, encode proteins of 251 and 250 residues that are 88% identical in overall amino acid sequence and 93% identical in the deduced mature protein. Each gene is present in a single copy per haploid genome; cab11 on chromosome 3 and cab12 on chromosome 6, and each has 2 introns located in similar positions to introns in other members of the Chl a/b-binding (CAB) protein gene family. Comparison of the amino acid sequences of LHCI, LHCII, CP29 and CP24 polypeptides confirms that all CABs share two regions of very high similarity which include the first and third transmembrane helices and the stroma-exposed sequences preceding them. However, near the N-terminus and between the conserved regions, the LHCI polypeptides have sequence motifs which appear to be PSI-specific.

Original languageEnglish (US)
Pages (from-to)229-234
Number of pages6
JournalFEBS Letters
Volume280
Issue number2
DOIs
StatePublished - Mar 25 1991
Externally publishedYes

Fingerprint

Photosystem I Protein Complex
Nucleotides
Genes
Antennas
Light
Peptides
Introns
Amino Acid Sequence
Chromosomes
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 3
Haploidy
Lycopersicon esculentum
Amino Acids
Cloning
Organism Cloning
Carrier Proteins
Proteins
Genome

Keywords

  • Chlorophyll a/b-(CAB) protein
  • Gene, cab11
  • Gene, cab12
  • Lycopersicon
  • Photosystem I light-harvesting complex

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Nucleotide sequence and chromosomal location of Cab11 and Cab12, the genes for the fourth polypeptide of the photosystem I light-harvesting antenna (LHCI). / Schwartz, Egbert; Shen, Dingren; Aebersold, Ruedi; McGrath, J. Mitchell; Pichersky, Eran; Green, Beverley R.

In: FEBS Letters, Vol. 280, No. 2, 25.03.1991, p. 229-234.

Research output: Contribution to journalArticle

Schwartz, Egbert ; Shen, Dingren ; Aebersold, Ruedi ; McGrath, J. Mitchell ; Pichersky, Eran ; Green, Beverley R. / Nucleotide sequence and chromosomal location of Cab11 and Cab12, the genes for the fourth polypeptide of the photosystem I light-harvesting antenna (LHCI). In: FEBS Letters. 1991 ; Vol. 280, No. 2. pp. 229-234.
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abstract = "Tryptic peptide sequences from the 22 kDa polypeptide of tomato LHCI were used to construct a probe for gene cloning. The two genes cloned, cab11 and cab12, encode proteins of 251 and 250 residues that are 88{\%} identical in overall amino acid sequence and 93{\%} identical in the deduced mature protein. Each gene is present in a single copy per haploid genome; cab11 on chromosome 3 and cab12 on chromosome 6, and each has 2 introns located in similar positions to introns in other members of the Chl a/b-binding (CAB) protein gene family. Comparison of the amino acid sequences of LHCI, LHCII, CP29 and CP24 polypeptides confirms that all CABs share two regions of very high similarity which include the first and third transmembrane helices and the stroma-exposed sequences preceding them. However, near the N-terminus and between the conserved regions, the LHCI polypeptides have sequence motifs which appear to be PSI-specific.",
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AU - McGrath, J. Mitchell

AU - Pichersky, Eran

AU - Green, Beverley R.

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AB - Tryptic peptide sequences from the 22 kDa polypeptide of tomato LHCI were used to construct a probe for gene cloning. The two genes cloned, cab11 and cab12, encode proteins of 251 and 250 residues that are 88% identical in overall amino acid sequence and 93% identical in the deduced mature protein. Each gene is present in a single copy per haploid genome; cab11 on chromosome 3 and cab12 on chromosome 6, and each has 2 introns located in similar positions to introns in other members of the Chl a/b-binding (CAB) protein gene family. Comparison of the amino acid sequences of LHCI, LHCII, CP29 and CP24 polypeptides confirms that all CABs share two regions of very high similarity which include the first and third transmembrane helices and the stroma-exposed sequences preceding them. However, near the N-terminus and between the conserved regions, the LHCI polypeptides have sequence motifs which appear to be PSI-specific.

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