Non-toxin-producing Bacillus cereus strains belonging to the B. anthracis clade isolated from the international space station

Kasthuri Venkateswaran, Nitin K. Singh, Aleksandra Checinska Sielaff, Robert K. Pope, Nicholas H. Bergman, Sandra P. Van Tongeren, Nisha B. Patel, Paul A. Lawson, Masataka Satomi, Charles H.D. Williamson, Jason W. Sahl, Paul S Keim, Duane Pierson, Jay Perry

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In an ongoing Microbial Observatory investigation of the International Space Station (ISS), 11 Bacillus strains (2 from the Kibo Japanese experimental module, 4 from the U.S. segment, and 5 from the Russian module) were isolated and their whole genomes were sequenced. A comparative analysis of the 16S rRNA gene sequences of these isolates showed the highest similarity (>99%) to the Bacillus anthracis- B. cereus-B. thuringiensis group. The fatty acid composition, polar lipid profile, peptidoglycan type, and matrix-assisted laser desorption ionization-time of flight profiles were consistent with the B. cereus sensu lato group. The phenotypic traits such as motile rods, enterotoxin production, lack of capsule, and resistance to gamma phage/penicillin observed in ISS isolates were not characteristics of B. anthracis. Whole-genome sequence characterizations showed that ISS strains had the plcR non-B. anthracis ancestral "C" allele and lacked anthrax toxin-encoding plasmids pXO1 and pXO2, excluding their identification as B. anthracis. The genetic identities of all 11 ISS isolates characterized via gyrB analyses arbitrarily identified them as members of the B. cereus group, but traditional DNA-DNA hybridization (DDH) showed that the ISS isolates are similar to B. anthracis (88% to 90%) but distant from the B. cereus (42%) and B. thuringiensis (48%) type strains. The DDH results were supported by average nucleotide identity (>98.5%) and digital DDH (>86%) analyses. However, the collective phenotypic traits and genomic evidence were the reasons to exclude the ISS isolates from B. anthracis. Nevertheless, multilocus sequence typing and whole-genome single nucleotide polymorphism analyses placed these isolates in a clade that is distinct from previously described members of the B. cereus sensu lato group but closely related to B. anthracis.

Original languageEnglish (US)
Article numbere00021
JournalmSystems
Volume2
Issue number3
DOIs
StatePublished - May 1 2017

Fingerprint

Bacillus cereus
Bacillus anthracis
Space stations
DNA
Genes
nucleic acid hybridization
Genome
genome
Bacilli
Nucleotides
anthrax
Multilocus Sequence Typing
Module
Bacteriophages
Single nucleotide Polymorphism
Peptidoglycan
Desorption
Enterotoxins
Time-of-flight
peptidoglycans

Keywords

  • Bacillus
  • Bacillus anthracis
  • Bacillus cereus
  • Genomics
  • ISS
  • Spores

ASJC Scopus subject areas

  • Molecular Biology
  • Physiology
  • Genetics
  • Biochemistry
  • Modeling and Simulation
  • Computer Science Applications
  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Cite this

Venkateswaran, K., Singh, N. K., Sielaff, A. C., Pope, R. K., Bergman, N. H., Van Tongeren, S. P., ... Perry, J. (2017). Non-toxin-producing Bacillus cereus strains belonging to the B. anthracis clade isolated from the international space station. mSystems, 2(3), [e00021]. https://doi.org/10.1128/mSystems.00021-17

Non-toxin-producing Bacillus cereus strains belonging to the B. anthracis clade isolated from the international space station. / Venkateswaran, Kasthuri; Singh, Nitin K.; Sielaff, Aleksandra Checinska; Pope, Robert K.; Bergman, Nicholas H.; Van Tongeren, Sandra P.; Patel, Nisha B.; Lawson, Paul A.; Satomi, Masataka; Williamson, Charles H.D.; Sahl, Jason W.; Keim, Paul S; Pierson, Duane; Perry, Jay.

In: mSystems, Vol. 2, No. 3, e00021, 01.05.2017.

Research output: Contribution to journalArticle

Venkateswaran, K, Singh, NK, Sielaff, AC, Pope, RK, Bergman, NH, Van Tongeren, SP, Patel, NB, Lawson, PA, Satomi, M, Williamson, CHD, Sahl, JW, Keim, PS, Pierson, D & Perry, J 2017, 'Non-toxin-producing Bacillus cereus strains belonging to the B. anthracis clade isolated from the international space station', mSystems, vol. 2, no. 3, e00021. https://doi.org/10.1128/mSystems.00021-17
Venkateswaran, Kasthuri ; Singh, Nitin K. ; Sielaff, Aleksandra Checinska ; Pope, Robert K. ; Bergman, Nicholas H. ; Van Tongeren, Sandra P. ; Patel, Nisha B. ; Lawson, Paul A. ; Satomi, Masataka ; Williamson, Charles H.D. ; Sahl, Jason W. ; Keim, Paul S ; Pierson, Duane ; Perry, Jay. / Non-toxin-producing Bacillus cereus strains belonging to the B. anthracis clade isolated from the international space station. In: mSystems. 2017 ; Vol. 2, No. 3.
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