Next-generation sequencing of Coccidioides immitis isolated during cluster investigation

David M. Engelthaler, Tom Chiller, James A. Schupp, Joshua Colvin, Stephen M Beckstrom-Sternberg, Elizabeth M. Driebe, Tracy Moses, Waibhav Tembe, Shripad Sinari, James S. Beckstrom-Sternberg, Alexis Christoforides, John V. Pearson, John Carpten, Paul S Keim, Ashley Peterson, Dawn Terashita, S. Arunmozhi Balajee

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 singlenucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalEmerging Infectious Diseases
Volume17
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

Cite this

Engelthaler, D. M., Chiller, T., Schupp, J. A., Colvin, J., Beckstrom-Sternberg, S. M., Driebe, E. M., Moses, T., Tembe, W., Sinari, S., Beckstrom-Sternberg, J. S., Christoforides, A., Pearson, J. V., Carpten, J., Keim, P. S., Peterson, A., Terashita, D., & Arunmozhi Balajee, S. (2011). Next-generation sequencing of Coccidioides immitis isolated during cluster investigation. Emerging Infectious Diseases, 17(2), 227-232. https://doi.org/10.3201/eid1702.100620