Nematospiroides dubius

Influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms

Fernando P Monroy, J. H. Adams, C. Dobson, I. J. East

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Five adjuvants were examined for their ability to potentiate the immune response of mice to soluble antigens from adult Nematospiroides dubius prepared by affinity chromatography against antibodies from repeatedly infected mice as ligands (IMIgAg). Immunized mice were better protected against N. dubius by IMIgAg injected intraperitoneally with either pertussigen (75%) or aluminium hydroxide (Alum) (67%) as adjuvants than with Freund's complete (54%) or incomplete adjuvants (31%). Protection was correlated with elevated specific antibody values and with cellular responses. Quil A was toxic to recipient mice at the concentration used. Alum may be a more practical adjuvant than pertussigen, which may activate protective immunity only in specific recipient genotypes and oil-based adjuvants which appear to be less efficient, to vaccinate mice with soluble parasite antigens.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalExperimental Parasitology
Volume68
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Nematospiroides dubius
Affinity Chromatography
Immunity
Antigens
Aluminum Hydroxide
Pertussis Toxin
Freund's Adjuvant
Antibodies
Poisons
Oils
Parasites
Genotype
Ligands

Keywords

  • Adjuvants
  • Immunity
  • Nematode
  • Nematospiroides dubius
  • Parasite antigens
  • Vaccines

ASJC Scopus subject areas

  • Immunology
  • Parasitology
  • Infectious Diseases

Cite this

Nematospiroides dubius : Influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms. / Monroy, Fernando P; Adams, J. H.; Dobson, C.; East, I. J.

In: Experimental Parasitology, Vol. 68, No. 1, 1989, p. 67-73.

Research output: Contribution to journalArticle

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