Nematospiroides dubius: Influence of adjuvants on immunity in mice vaccinated with antigens isolated by affinity chromatography from adult worms

F. G. Monroy, J. H. Adams, C. Dobson, I. J. East

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Five adjuvants were examined for their ability to potentiate the immune response of mice to soluble antigens from adult Nematospiroides dubius prepared by affinity chromatography against antibodies from repeatedly infected mice as ligands (IMIgAg). Immunized mice were better protected against N. dubius by IMIgAg injected intraperitoneally with either pertussigen (75%) or aluminium hydroxide (Alum) (67%) as adjuvants than with Freund's complete (54%) or incomplete adjuvants (31%). Protection was correlated with elevated specific antibody values and with cellular responses. Quil A was toxic to recipient mice at the concentration used. Alum may be a more practical adjuvant than pertussigen, which may activate protective immunity only in specific recipient genotypes and oil-based adjuvants which appear to be less efficient, to vaccinate mice with soluble parasite antigens.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalExperimental Parasitology
Volume68
Issue number1
DOIs
StatePublished - Jan 1989

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Keywords

  • Adjuvants
  • Immunity
  • Nematode
  • Nematospiroides dubius
  • Parasite antigens
  • Vaccines

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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