Myosin heavy chain mRNA and protein expression in single fibers of the rat soleus following reinnervation

Sue C. Bodine, David J Pierotti

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Myosin heavy chain (MHC) expression is regulated by many factors including neural input. To gain a better understanding of myosin transformation following reinnervation we examined both MHC protein and mRNA in single fibers of the soleus. A midthigh sciatic nerve lesion resulted in reinnervation of the soleus by motoneurons from both the original and foreign motor pools. MHC expression was examined in individual fibers 8 and 16 weeks post injury using in situ histochemistry and immunohistochemistry. Following a sciatic nerve lesion, the reinnervated soleus underwent a transformation from slow toward fast based on physiologic and biochemical measurements. At 8 weeks, fast MHC mRNA isoforms (IIa and IIx) were upregulated and slow mRNA was downregulated, however, the predominant protein isoform was MHC I. At both 8 and 16 weeks, many fibers expressed multiple mRNA isoforms. At 16 weeks there was limited co-expression of slow and fast MHC mRNAs, but continued co-expression of fast MHC mRNAs. Sixteen weeks following reinnervation the predominant fast mRNA and protein in the soleus was IIx MHC.

Original languageEnglish (US)
Pages (from-to)13-16
Number of pages4
JournalNeuroscience Letters
Volume215
Issue number1
DOIs
StatePublished - Aug 30 1996
Externally publishedYes

Fingerprint

Myosin Heavy Chains
Messenger RNA
Proteins
Sciatic Neuropathy
RNA Isoforms
Motor Neurons
Myosins
Protein Isoforms
Down-Regulation
Immunohistochemistry
Wounds and Injuries

Keywords

  • Denervation
  • Immunohistochemistry
  • In situ histochemistry
  • Myosin heavy chain gene regulation
  • Sciatic nerve injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Myosin heavy chain mRNA and protein expression in single fibers of the rat soleus following reinnervation. / Bodine, Sue C.; Pierotti, David J.

In: Neuroscience Letters, Vol. 215, No. 1, 30.08.1996, p. 13-16.

Research output: Contribution to journalArticle

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N2 - Myosin heavy chain (MHC) expression is regulated by many factors including neural input. To gain a better understanding of myosin transformation following reinnervation we examined both MHC protein and mRNA in single fibers of the soleus. A midthigh sciatic nerve lesion resulted in reinnervation of the soleus by motoneurons from both the original and foreign motor pools. MHC expression was examined in individual fibers 8 and 16 weeks post injury using in situ histochemistry and immunohistochemistry. Following a sciatic nerve lesion, the reinnervated soleus underwent a transformation from slow toward fast based on physiologic and biochemical measurements. At 8 weeks, fast MHC mRNA isoforms (IIa and IIx) were upregulated and slow mRNA was downregulated, however, the predominant protein isoform was MHC I. At both 8 and 16 weeks, many fibers expressed multiple mRNA isoforms. At 16 weeks there was limited co-expression of slow and fast MHC mRNAs, but continued co-expression of fast MHC mRNAs. Sixteen weeks following reinnervation the predominant fast mRNA and protein in the soleus was IIx MHC.

AB - Myosin heavy chain (MHC) expression is regulated by many factors including neural input. To gain a better understanding of myosin transformation following reinnervation we examined both MHC protein and mRNA in single fibers of the soleus. A midthigh sciatic nerve lesion resulted in reinnervation of the soleus by motoneurons from both the original and foreign motor pools. MHC expression was examined in individual fibers 8 and 16 weeks post injury using in situ histochemistry and immunohistochemistry. Following a sciatic nerve lesion, the reinnervated soleus underwent a transformation from slow toward fast based on physiologic and biochemical measurements. At 8 weeks, fast MHC mRNA isoforms (IIa and IIx) were upregulated and slow mRNA was downregulated, however, the predominant protein isoform was MHC I. At both 8 and 16 weeks, many fibers expressed multiple mRNA isoforms. At 16 weeks there was limited co-expression of slow and fast MHC mRNAs, but continued co-expression of fast MHC mRNAs. Sixteen weeks following reinnervation the predominant fast mRNA and protein in the soleus was IIx MHC.

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