More than 50% of clostridium difficile isolates from pet dogs in Flagstaff, USA, carry toxigenic genotypes

Nathan E. Stone, Lindsay C. Sidak-Loftis, Jason W. Sahl, Adam J. Vazquez, Kristin B. Wiggins, John D. Gillece, Nathan D. Hicks, James M. Schupp, Joseph D. Busch, Paul S Keim, David M Wagner

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Nosocomial acquisition of Clostridium difficile is well documented, yet recent studies have highlighted the importance of community acquired infections and identified community associated reservoirs for this pathogen. Multiple studies have implicated companion pets and farm animals as possible sources of community acquired C. difficile infections in humans. To explore the potential role of pet dogs in human C. difficile infections we systematically collected canine fecal samples (n = 197) in Flagstaff, AZ. Additionally, nineteen fecal samples were collected at a local veterinary clinic from diarrheic dogs. We used these combined samples to investigate important questions regarding C. difficile colonization in pet canines: 1) What is the prevalence and diversity of C. difficile in this companion pet population, and 2) Do C. difficile isolates collected from canines genetically overlap with isolates that cause disease in humans? We used a two-step sequence typing approach, including multilocus sequence typing to determine the overall genetic diversity of C. difficile present in Flagstaff canines, and whole-genome sequencing to assess the fine-scale diversity patterns within identical multilocus sequence types from isolates obtained within and among multiple canine hosts. We detected C. difficile in 17% of the canine fecal samples with 10% containing toxigenic strains that are known to cause human disease. Sequencing analyses revealed similar genotypes in dogs and humans. These findings suggest that companion pets are a potential source of community acquired C. difficile infections in humans.

Original languageEnglish (US)
Article numbere0164504
JournalPLoS One
Volume11
Issue number10
DOIs
StatePublished - Oct 1 2016

Fingerprint

Clostridium difficile
Clostridium
Pets
pets
Genotype
Dogs
Canidae
dogs
genotype
Pathogens
Farms
Clostridium Infections
Animals
Genes
human diseases
infection
Community-Acquired Infections
Animal Hospitals
Multilocus Sequence Typing
sampling

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Stone, N. E., Sidak-Loftis, L. C., Sahl, J. W., Vazquez, A. J., Wiggins, K. B., Gillece, J. D., ... Wagner, D. M. (2016). More than 50% of clostridium difficile isolates from pet dogs in Flagstaff, USA, carry toxigenic genotypes. PLoS One, 11(10), [e0164504]. https://doi.org/10.1371/journal.pone.0164504

More than 50% of clostridium difficile isolates from pet dogs in Flagstaff, USA, carry toxigenic genotypes. / Stone, Nathan E.; Sidak-Loftis, Lindsay C.; Sahl, Jason W.; Vazquez, Adam J.; Wiggins, Kristin B.; Gillece, John D.; Hicks, Nathan D.; Schupp, James M.; Busch, Joseph D.; Keim, Paul S; Wagner, David M.

In: PLoS One, Vol. 11, No. 10, e0164504, 01.10.2016.

Research output: Contribution to journalArticle

Stone, NE, Sidak-Loftis, LC, Sahl, JW, Vazquez, AJ, Wiggins, KB, Gillece, JD, Hicks, ND, Schupp, JM, Busch, JD, Keim, PS & Wagner, DM 2016, 'More than 50% of clostridium difficile isolates from pet dogs in Flagstaff, USA, carry toxigenic genotypes', PLoS One, vol. 11, no. 10, e0164504. https://doi.org/10.1371/journal.pone.0164504
Stone NE, Sidak-Loftis LC, Sahl JW, Vazquez AJ, Wiggins KB, Gillece JD et al. More than 50% of clostridium difficile isolates from pet dogs in Flagstaff, USA, carry toxigenic genotypes. PLoS One. 2016 Oct 1;11(10). e0164504. https://doi.org/10.1371/journal.pone.0164504
Stone, Nathan E. ; Sidak-Loftis, Lindsay C. ; Sahl, Jason W. ; Vazquez, Adam J. ; Wiggins, Kristin B. ; Gillece, John D. ; Hicks, Nathan D. ; Schupp, James M. ; Busch, Joseph D. ; Keim, Paul S ; Wagner, David M. / More than 50% of clostridium difficile isolates from pet dogs in Flagstaff, USA, carry toxigenic genotypes. In: PLoS One. 2016 ; Vol. 11, No. 10.
@article{d482ab1f1e074b86a70b4f2a8d64bd5a,
title = "More than 50{\%} of clostridium difficile isolates from pet dogs in Flagstaff, USA, carry toxigenic genotypes",
abstract = "Nosocomial acquisition of Clostridium difficile is well documented, yet recent studies have highlighted the importance of community acquired infections and identified community associated reservoirs for this pathogen. Multiple studies have implicated companion pets and farm animals as possible sources of community acquired C. difficile infections in humans. To explore the potential role of pet dogs in human C. difficile infections we systematically collected canine fecal samples (n = 197) in Flagstaff, AZ. Additionally, nineteen fecal samples were collected at a local veterinary clinic from diarrheic dogs. We used these combined samples to investigate important questions regarding C. difficile colonization in pet canines: 1) What is the prevalence and diversity of C. difficile in this companion pet population, and 2) Do C. difficile isolates collected from canines genetically overlap with isolates that cause disease in humans? We used a two-step sequence typing approach, including multilocus sequence typing to determine the overall genetic diversity of C. difficile present in Flagstaff canines, and whole-genome sequencing to assess the fine-scale diversity patterns within identical multilocus sequence types from isolates obtained within and among multiple canine hosts. We detected C. difficile in 17{\%} of the canine fecal samples with 10{\%} containing toxigenic strains that are known to cause human disease. Sequencing analyses revealed similar genotypes in dogs and humans. These findings suggest that companion pets are a potential source of community acquired C. difficile infections in humans.",
author = "Stone, {Nathan E.} and Sidak-Loftis, {Lindsay C.} and Sahl, {Jason W.} and Vazquez, {Adam J.} and Wiggins, {Kristin B.} and Gillece, {John D.} and Hicks, {Nathan D.} and Schupp, {James M.} and Busch, {Joseph D.} and Keim, {Paul S} and Wagner, {David M}",
year = "2016",
month = "10",
day = "1",
doi = "10.1371/journal.pone.0164504",
language = "English (US)",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

TY - JOUR

T1 - More than 50% of clostridium difficile isolates from pet dogs in Flagstaff, USA, carry toxigenic genotypes

AU - Stone, Nathan E.

AU - Sidak-Loftis, Lindsay C.

AU - Sahl, Jason W.

AU - Vazquez, Adam J.

AU - Wiggins, Kristin B.

AU - Gillece, John D.

AU - Hicks, Nathan D.

AU - Schupp, James M.

AU - Busch, Joseph D.

AU - Keim, Paul S

AU - Wagner, David M

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Nosocomial acquisition of Clostridium difficile is well documented, yet recent studies have highlighted the importance of community acquired infections and identified community associated reservoirs for this pathogen. Multiple studies have implicated companion pets and farm animals as possible sources of community acquired C. difficile infections in humans. To explore the potential role of pet dogs in human C. difficile infections we systematically collected canine fecal samples (n = 197) in Flagstaff, AZ. Additionally, nineteen fecal samples were collected at a local veterinary clinic from diarrheic dogs. We used these combined samples to investigate important questions regarding C. difficile colonization in pet canines: 1) What is the prevalence and diversity of C. difficile in this companion pet population, and 2) Do C. difficile isolates collected from canines genetically overlap with isolates that cause disease in humans? We used a two-step sequence typing approach, including multilocus sequence typing to determine the overall genetic diversity of C. difficile present in Flagstaff canines, and whole-genome sequencing to assess the fine-scale diversity patterns within identical multilocus sequence types from isolates obtained within and among multiple canine hosts. We detected C. difficile in 17% of the canine fecal samples with 10% containing toxigenic strains that are known to cause human disease. Sequencing analyses revealed similar genotypes in dogs and humans. These findings suggest that companion pets are a potential source of community acquired C. difficile infections in humans.

AB - Nosocomial acquisition of Clostridium difficile is well documented, yet recent studies have highlighted the importance of community acquired infections and identified community associated reservoirs for this pathogen. Multiple studies have implicated companion pets and farm animals as possible sources of community acquired C. difficile infections in humans. To explore the potential role of pet dogs in human C. difficile infections we systematically collected canine fecal samples (n = 197) in Flagstaff, AZ. Additionally, nineteen fecal samples were collected at a local veterinary clinic from diarrheic dogs. We used these combined samples to investigate important questions regarding C. difficile colonization in pet canines: 1) What is the prevalence and diversity of C. difficile in this companion pet population, and 2) Do C. difficile isolates collected from canines genetically overlap with isolates that cause disease in humans? We used a two-step sequence typing approach, including multilocus sequence typing to determine the overall genetic diversity of C. difficile present in Flagstaff canines, and whole-genome sequencing to assess the fine-scale diversity patterns within identical multilocus sequence types from isolates obtained within and among multiple canine hosts. We detected C. difficile in 17% of the canine fecal samples with 10% containing toxigenic strains that are known to cause human disease. Sequencing analyses revealed similar genotypes in dogs and humans. These findings suggest that companion pets are a potential source of community acquired C. difficile infections in humans.

UR - http://www.scopus.com/inward/record.url?scp=84991516771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991516771&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0164504

DO - 10.1371/journal.pone.0164504

M3 - Article

VL - 11

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 10

M1 - e0164504

ER -