Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea

Anthony Baker, Talima R Pearson, Erin P. Price, Julia Dale, Paul S Keim, Heidie Hornstra, Andrew Greenhill, Gabriel Padilla, Jeffrey Warner

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: The island of New Guinea is located midway between the world's two major melioidosis endemic regions of Australia and Southeast Asia. Previous studies in Papua New Guinea have demonstrated autochthonous melioidosis in Balimo, Western province. In contrast to other regions of endemicity, isolates recovered from both environmental and clinical sources demonstrate narrow genetic diversity over large spatial and temporal scales. Methodology/Principal Findings: We employed molecular typing techniques to determine the phylogenetic relationships of these isolates to each other and to others worldwide to aid in understanding the origins of the Papua New Guinean isolates. Multi-locus sequence typing of the 39 isolates resolved three unique sequence types. Phylogenetic reconstruction and Structure analysis determined that all isolates were genetically closer to those from Australia than those from Southeast Asia. Gene cluster analysis however, identified a Yersinia-like fimbrial gene cluster predominantly found among Burkholderia pseudomallei derived from Southeast Asia. Higher resolution VNTR typing and phylogenetic reconstruction of the Balimo isolates resolved 24 genotypes with long branch lengths. These findings are congruent with long term persistence in the region and a high level of environmental stability. Conclusions/Significance: Given that anthropogenic influence has been hypothesized as a mechanism for the dispersal of B. pseudomallei, these findings correlate with limited movement of the indigenous people in the region. The palaeogeographical and anthropogenic history of Australasia and the results from this study indicate that New Guinea is an important region for the further study of B. pseudomallei origins and dissemination.

Original languageEnglish (US)
Article numbere18343
JournalPLoS One
Volume6
Issue number3
DOIs
StatePublished - 2011

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Burkholderia pseudomallei
Papua New Guinea
Southeastern Asia
Phylogeny
South East Asia
New Guinea
Melioidosis
Genes
Multigene Family
multigene family
phylogeny
Cluster analysis
Australasia
Molecular Typing
Yersinia
Australasian region
indigenous peoples
minisatellite repeats
Islands
anthropogenic activities

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea. / Baker, Anthony; Pearson, Talima R; Price, Erin P.; Dale, Julia; Keim, Paul S; Hornstra, Heidie; Greenhill, Andrew; Padilla, Gabriel; Warner, Jeffrey.

In: PLoS One, Vol. 6, No. 3, e18343, 2011.

Research output: Contribution to journalArticle

Baker, A, Pearson, TR, Price, EP, Dale, J, Keim, PS, Hornstra, H, Greenhill, A, Padilla, G & Warner, J 2011, 'Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea', PLoS One, vol. 6, no. 3, e18343. https://doi.org/10.1371/journal.pone.0018343
Baker, Anthony ; Pearson, Talima R ; Price, Erin P. ; Dale, Julia ; Keim, Paul S ; Hornstra, Heidie ; Greenhill, Andrew ; Padilla, Gabriel ; Warner, Jeffrey. / Molecular phylogeny of Burkholderia pseudomallei from a remote region of Papua New Guinea. In: PLoS One. 2011 ; Vol. 6, No. 3.
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