Molecular evolution of gypc

Evidence for recent structural innovation and positive selection in humans

Jason A. Wilder, Elizabeth K. Hewett, Meredith E. Gansner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

GYPC encodes two erythrocyte surface sialoglycoproteins in humans, glycophorin C and glycophorin D (GPC and GPD), via initiation of translation at two start codons on a single transcript. The malaria-causing parasite Plasmodium falciparum uses GPC as a means of invasion into the human red blood cell. Here, we examine the molecular evolution of GYPC among the Hominoidea (Greater and Lesser Apes) and also the pattern of polymorphism at the locus in a global human sample. We find an excess of nonsynonymous divergence among species that appears to be caused solely by accelerated evolution of GYPC in the human lineage. Moreover, we find that the ability of GYPC to encode both GPC and GPD is a uniquely human trait, caused by the evolution of the GPC start codon in the human lineage. The pattern of polymorphism among humans is consistent with a hitchhiking event at the locus, suggesting that positive natural selection affected GYPC in the relatively recent past. Because GPC is exploited by P. falciparum for invasion of the red blood cell, we hypothesize that selection for evasion of P. falciparum has caused accelerated evolution of GYPC in humans (relative to other primates) and that this positive selection has continued to act in the recent evolution of our species. These data suggest that malaria has played a powerful role in shaping molecules on the surface of the human red blood cell. In addition, our examination of GYPC reveals a novel mechanism of protein evolution: co-option of untranslated region (UTR) sequence following the formation of a new start codon. In the case of human GYPC, the ancestral protein (GPD) continues to be produced through leaky translation. Because leaky translation is a widespread phenomenon among genes and organisms, we suggest that co-option of UTR sequence may be an important source of protein innovation.

Original languageEnglish (US)
Pages (from-to)2679-2687
Number of pages9
JournalMolecular Biology and Evolution
Volume26
Issue number12
DOIs
StatePublished - Dec 2009

Fingerprint

Molecular Evolution
innovation
Glycophorin
blood
malaria
start codon
Initiator Codon
Plasmodium falciparum
erythrocytes
protein
translation (genetics)
polymorphism
Untranslated Regions
Erythrocytes
Malaria
natural selection
primate
genetic polymorphism
parasite
divergence

Keywords

  • GYPC
  • Leaky translation
  • Positive selection
  • Sialoglycoproteins
  • UTR capture

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Molecular evolution of gypc : Evidence for recent structural innovation and positive selection in humans. / Wilder, Jason A.; Hewett, Elizabeth K.; Gansner, Meredith E.

In: Molecular Biology and Evolution, Vol. 26, No. 12, 12.2009, p. 2679-2687.

Research output: Contribution to journalArticle

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