Molecular epidemiology of anthrax cases associated with recreational use of animal hides and yarn in the United States

Chung K. Marston, Christina A. Allen, Jodi Beaudry, Erin P. Price, Spenser R. Wolken, Talima R Pearson, Paul S Keim, Alex R. Hoffmaster

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

To determine potential links between the clinical isolate to animal products and their geographic origin, we genotyped (MLVA-8, MVLA-15, and canSNP analysis) 80 environmental and 12 clinical isolates and 2 clinical specimens from five cases of anthrax (California in 1976 [n = 1], New York in 2006 [n = 1], Connecticut in 2007 [n = 2], and New Hampshire in 2009[n = 1]) resulting from recreational handling of animal products. For the California case, four clinical isolates were identified as MLVA-8 genotype (GT) 76 and in the canSNP A.Br.Vollum lineage, which is consistent with the Pakistani origin of the yarn. Twenty eight of the California isolates were in the A.Br.Vollum canSNP lineage and one isolate was in the A.Br. 003/004 canSNP sub-group. All 52 isolates and both clinical specimens related to the New York and Connecticut cases were MLVA-8 GT 1. The animal products associated with the NY and CT cases were believed to originate from West Africa, but no isolates from this region are available to be genotyped for comparison. All isolates associated with the New Hampshire case were identical and had a new genotype (GT 149). Isolates from the NY, CT and NH cases diverge from the established canSNP phylogeny near the base of the A.Br.011/009. This report illustrates the power of the current genotyping methods and the dramatically different epidemiological conditions that can lead to infections (i.e., contamination by a single genotype versus widespread contamination of numerous genotypes). These cases illustrate the need to acquire and genotype global isolates so that accurate assignments can be made about isolate origins.

Original languageEnglish (US)
Article numbere28274
JournalPLoS One
Volume6
Issue number12
DOIs
StatePublished - Dec 9 2011

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Anthrax
anthrax
molecular epidemiology
Molecular Epidemiology
yarns
Yarn
Animals
Genotype
genotype
Contamination
animal products
animals
Western Africa
Phylogeny
provenance
genotyping
phylogeny
Infection
infection

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Molecular epidemiology of anthrax cases associated with recreational use of animal hides and yarn in the United States. / Marston, Chung K.; Allen, Christina A.; Beaudry, Jodi; Price, Erin P.; Wolken, Spenser R.; Pearson, Talima R; Keim, Paul S; Hoffmaster, Alex R.

In: PLoS One, Vol. 6, No. 12, e28274, 09.12.2011.

Research output: Contribution to journalArticle

Marston, Chung K. ; Allen, Christina A. ; Beaudry, Jodi ; Price, Erin P. ; Wolken, Spenser R. ; Pearson, Talima R ; Keim, Paul S ; Hoffmaster, Alex R. / Molecular epidemiology of anthrax cases associated with recreational use of animal hides and yarn in the United States. In: PLoS One. 2011 ; Vol. 6, No. 12.
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