Molecular diversity in Bacillus anthracis

Paul S Keim, A. M. Klevytska, L. B. Price, J. M. Schupp, G. Zinser, K. L. Smith, M. E. Hugh-Jones, R. Okinaka, K. K. Hill, P. J. Jackson

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Molecular typing of Bacillus anthracis has been extremely difficult due to the lack of polymorphic DNA markers. We have identified nine novel variable number tandemly repeated loci from previously known amplified fragment length polymorphism markers or from the DNA sequence. In combination with the previously known vrrA locus, these markers provide discrimination power to genetically characterize B, anthracis isolates. The variable number tandem repeat (VNTR) loci are found in both gene coding (genie) and non- coding (non-genic) regions. The genic differences are 'in frame' and result in additions or deletion of amino acids to the predicted proteins. Due the rarity of molecular differences, the VNTR changes represent a significant portion of the genetic variation found within B. anthracis. This variation could represent an important adaptive mechanism. Marker similarity and differences among diverse isolates have identified seven major diversity groups that may represent the only world-wide B. anthracis clones. The lineages reconstructed using these data may reflect the dispersal and evolution of this pathogen.

Original languageEnglish (US)
Pages (from-to)215-217
Number of pages3
JournalJournal of Applied Microbiology
Volume87
Issue number2
DOIs
StatePublished - 1999

Fingerprint

Bacillus anthracis
Minisatellite Repeats
minisatellite repeats
loci
amino acid deletion
Molecular Typing
Genetic Markers
amplified fragment length polymorphism
Clone Cells
clones
nucleotide sequences
Amino Acids
genetic variation
genetic markers
pathogens
Genes
Proteins
genes
proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Keim, P. S., Klevytska, A. M., Price, L. B., Schupp, J. M., Zinser, G., Smith, K. L., ... Jackson, P. J. (1999). Molecular diversity in Bacillus anthracis. Journal of Applied Microbiology, 87(2), 215-217. https://doi.org/10.1046/j.1365-2672.1999.00873.x

Molecular diversity in Bacillus anthracis. / Keim, Paul S; Klevytska, A. M.; Price, L. B.; Schupp, J. M.; Zinser, G.; Smith, K. L.; Hugh-Jones, M. E.; Okinaka, R.; Hill, K. K.; Jackson, P. J.

In: Journal of Applied Microbiology, Vol. 87, No. 2, 1999, p. 215-217.

Research output: Contribution to journalArticle

Keim, PS, Klevytska, AM, Price, LB, Schupp, JM, Zinser, G, Smith, KL, Hugh-Jones, ME, Okinaka, R, Hill, KK & Jackson, PJ 1999, 'Molecular diversity in Bacillus anthracis', Journal of Applied Microbiology, vol. 87, no. 2, pp. 215-217. https://doi.org/10.1046/j.1365-2672.1999.00873.x
Keim PS, Klevytska AM, Price LB, Schupp JM, Zinser G, Smith KL et al. Molecular diversity in Bacillus anthracis. Journal of Applied Microbiology. 1999;87(2):215-217. https://doi.org/10.1046/j.1365-2672.1999.00873.x
Keim, Paul S ; Klevytska, A. M. ; Price, L. B. ; Schupp, J. M. ; Zinser, G. ; Smith, K. L. ; Hugh-Jones, M. E. ; Okinaka, R. ; Hill, K. K. ; Jackson, P. J. / Molecular diversity in Bacillus anthracis. In: Journal of Applied Microbiology. 1999 ; Vol. 87, No. 2. pp. 215-217.
@article{26ffcf31315e480883bfde4292dc11ae,
title = "Molecular diversity in Bacillus anthracis",
abstract = "Molecular typing of Bacillus anthracis has been extremely difficult due to the lack of polymorphic DNA markers. We have identified nine novel variable number tandemly repeated loci from previously known amplified fragment length polymorphism markers or from the DNA sequence. In combination with the previously known vrrA locus, these markers provide discrimination power to genetically characterize B, anthracis isolates. The variable number tandem repeat (VNTR) loci are found in both gene coding (genie) and non- coding (non-genic) regions. The genic differences are 'in frame' and result in additions or deletion of amino acids to the predicted proteins. Due the rarity of molecular differences, the VNTR changes represent a significant portion of the genetic variation found within B. anthracis. This variation could represent an important adaptive mechanism. Marker similarity and differences among diverse isolates have identified seven major diversity groups that may represent the only world-wide B. anthracis clones. The lineages reconstructed using these data may reflect the dispersal and evolution of this pathogen.",
author = "Keim, {Paul S} and Klevytska, {A. M.} and Price, {L. B.} and Schupp, {J. M.} and G. Zinser and Smith, {K. L.} and Hugh-Jones, {M. E.} and R. Okinaka and Hill, {K. K.} and Jackson, {P. J.}",
year = "1999",
doi = "10.1046/j.1365-2672.1999.00873.x",
language = "English (US)",
volume = "87",
pages = "215--217",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Molecular diversity in Bacillus anthracis

AU - Keim, Paul S

AU - Klevytska, A. M.

AU - Price, L. B.

AU - Schupp, J. M.

AU - Zinser, G.

AU - Smith, K. L.

AU - Hugh-Jones, M. E.

AU - Okinaka, R.

AU - Hill, K. K.

AU - Jackson, P. J.

PY - 1999

Y1 - 1999

N2 - Molecular typing of Bacillus anthracis has been extremely difficult due to the lack of polymorphic DNA markers. We have identified nine novel variable number tandemly repeated loci from previously known amplified fragment length polymorphism markers or from the DNA sequence. In combination with the previously known vrrA locus, these markers provide discrimination power to genetically characterize B, anthracis isolates. The variable number tandem repeat (VNTR) loci are found in both gene coding (genie) and non- coding (non-genic) regions. The genic differences are 'in frame' and result in additions or deletion of amino acids to the predicted proteins. Due the rarity of molecular differences, the VNTR changes represent a significant portion of the genetic variation found within B. anthracis. This variation could represent an important adaptive mechanism. Marker similarity and differences among diverse isolates have identified seven major diversity groups that may represent the only world-wide B. anthracis clones. The lineages reconstructed using these data may reflect the dispersal and evolution of this pathogen.

AB - Molecular typing of Bacillus anthracis has been extremely difficult due to the lack of polymorphic DNA markers. We have identified nine novel variable number tandemly repeated loci from previously known amplified fragment length polymorphism markers or from the DNA sequence. In combination with the previously known vrrA locus, these markers provide discrimination power to genetically characterize B, anthracis isolates. The variable number tandem repeat (VNTR) loci are found in both gene coding (genie) and non- coding (non-genic) regions. The genic differences are 'in frame' and result in additions or deletion of amino acids to the predicted proteins. Due the rarity of molecular differences, the VNTR changes represent a significant portion of the genetic variation found within B. anthracis. This variation could represent an important adaptive mechanism. Marker similarity and differences among diverse isolates have identified seven major diversity groups that may represent the only world-wide B. anthracis clones. The lineages reconstructed using these data may reflect the dispersal and evolution of this pathogen.

UR - http://www.scopus.com/inward/record.url?scp=0032847302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032847302&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2672.1999.00873.x

DO - 10.1046/j.1365-2672.1999.00873.x

M3 - Article

C2 - 10475952

AN - SCOPUS:0032847302

VL - 87

SP - 215

EP - 217

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 2

ER -