Molecular detection of the causative agent of white-nose syndrome on rafinesque’s big-eared bats (Corynorhinus rafinesquii) and two species of migratory bats in the southeastern USA

Riley F. Bernard, Jeffrey T Foster, Emma V. Willcox, Katy L. Parise, Gary F. McCracken

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pseudogymnoascus destructans, the causal agent of white-nose syndrome (WNS), is responsible for widespread mortality of hibernating bats across eastern North America. To document P. destructans exposure and infections on bats active during winter in the southeastern US, we collected epidermal swabs from bats captured during winters 2012-13 and 2013-14 in mist nets set outside of hibernacula in Tennessee. Epidermal swab samples were collected from eight Rafinesque’s big-eared bats (Corynorhinus rafinesquii), six eastern red bats (Lasiurus borealis), and three silver-hair bats (Lasionycteris noctivagans). Using real-time PCR methods, we identified DNA sequences of P. destructans from skin swabs of two Rafinesque’s big-eared bats, two eastern red bats, and one silver-haired bat. This is the first detection of the WNS fungus on Rafinesque’s big-eared bats and eastern red bats and the second record of the presence of the fungus on silver-haired bats.

Original languageEnglish (US)
Pages (from-to)519-522
Number of pages4
JournalJournal of Wildlife Diseases
Volume51
Issue number2
DOIs
StatePublished - 2015

Fingerprint

bat
Chiroptera
Lasiurus borealis
silver
migratory species
white-nose syndrome
detection
fungi
winter
fungus
hibernation
skin (animal)
hairs
quantitative polymerase chain reaction
hair
nucleotide sequences
skin
DNA
mortality
infection

Keywords

  • Bats
  • Fungus
  • Pseudogymnoascus destructans
  • Swabs
  • White-nose syndrome
  • Winter

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

Molecular detection of the causative agent of white-nose syndrome on rafinesque’s big-eared bats (Corynorhinus rafinesquii) and two species of migratory bats in the southeastern USA. / Bernard, Riley F.; Foster, Jeffrey T; Willcox, Emma V.; Parise, Katy L.; McCracken, Gary F.

In: Journal of Wildlife Diseases, Vol. 51, No. 2, 2015, p. 519-522.

Research output: Contribution to journalArticle

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