Molecular analysis of rifampin resistance in Bacillus anthracis and Bacillus cereus

Amy J. Vogler, Joseph D. Busch, Stephanie Percy-Fine, Christine Tipton-Hunton, Kimothy L. Smith, Paul S Keim

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Rifampin-resistant mutants were selected from UV-light-treated Bacillus cereus (20 mutants) and attenuated B. anthracis (23 mutants). In addition, spontaneous rifampin-resistant mutants were also isolated in attenuated B. anthracis (22 mutants). The rifampin resistance clusters of the rpoB gene were sequenced for all 65 mutants. Mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. The spontaneous rate of resistance was estimated at 1.57 × 10-9 mutations/generation by a Luria-Delbrück fluctuation test.

Original languageEnglish (US)
Pages (from-to)511-513
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Issue number2
StatePublished - 2002


ASJC Scopus subject areas

  • Pharmacology (medical)

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