Modeling cell line-specific recruitment of signaling proteins to the insulin-like growth factor 1 receptor

Keesha E. Erickson, Oleksii S. Rukhlenko, Md Shahinuzzaman, Kalina P. Slavkova, Yen Ting Lin, Ryan Suderman, Edward C. Stites, Marian Anghel, Richard G Posner, Dipak Barua, Boris N. Kholodenko, William S. Hlavacek

Research output: Contribution to journalArticle

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Abstract

Receptor tyrosine kinases (RTKs) typically contain multiple autophosphorylation sites in their cytoplasmic domains. Once activated, these autophosphorylation sites can recruit downstream signaling proteins containing Src homology 2 (SH2) and phosphotyrosine-binding (PTB) domains, which recognize phosphotyrosine-containing short linear motifs (SLiMs). These domains and SLiMs have polyspecific or promiscuous binding activities. Thus, multiple signaling proteins may compete for binding to a common SLiM and vice versa. To investigate the effects of competition on RTK signaling, we used a rule-based modeling approach to develop and analyze models for ligand-induced recruitment of SH2/PTB domain-containing proteins to autophosphorylation sites in the insulin-like growth factor 1 (IGF1) receptor (IGF1R). Models were parameterized using published datasets reporting protein copy numbers and site-specific binding affinities. Simulations were facilitated by a novel application of model restructuration, to reduce redundancy in rule-derived equations. We compare predictions obtained via numerical simulation of the model to those obtained through simple prediction methods, such as through an analytical approximation, or ranking by copy number and/or KD value, and find that the simple methods are unable to recapitulate the predictions of numerical simulations. We created 45 cell line-specific models that demonstrate how early events in IGF1R signaling depend on the protein abundance profile of a cell. Simulations, facilitated by model restructuration, identified pairs of IGF1R binding partners that are recruited in anti-correlated and correlated fashions, despite no inclusion of cooperativity in our models. This work shows that the outcome of competition depends on the physicochemical parameters that characterize pairwise interactions, as well as network properties, including network connectivity and the relative abundances of competitors.

Original languageEnglish (US)
Pages (from-to)e1006706
JournalPLoS Computational Biology
Volume15
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Somatomedin Receptors
Insulin
Growth Factors
Receptor
Phosphotyrosine
Cells
cell lines
Proteins
Protein
Cell Line
protein
Line
Cell
Receptor Protein-Tyrosine Kinases
Modeling
protein phosphorylation
modeling
proteins
receptors
homology

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this

Erickson, K. E., Rukhlenko, O. S., Shahinuzzaman, M., Slavkova, K. P., Lin, Y. T., Suderman, R., ... Hlavacek, W. S. (2019). Modeling cell line-specific recruitment of signaling proteins to the insulin-like growth factor 1 receptor. PLoS Computational Biology, 15(1), e1006706. https://doi.org/10.1371/journal.pcbi.1006706

Modeling cell line-specific recruitment of signaling proteins to the insulin-like growth factor 1 receptor. / Erickson, Keesha E.; Rukhlenko, Oleksii S.; Shahinuzzaman, Md; Slavkova, Kalina P.; Lin, Yen Ting; Suderman, Ryan; Stites, Edward C.; Anghel, Marian; Posner, Richard G; Barua, Dipak; Kholodenko, Boris N.; Hlavacek, William S.

In: PLoS Computational Biology, Vol. 15, No. 1, 01.01.2019, p. e1006706.

Research output: Contribution to journalArticle

Erickson, KE, Rukhlenko, OS, Shahinuzzaman, M, Slavkova, KP, Lin, YT, Suderman, R, Stites, EC, Anghel, M, Posner, RG, Barua, D, Kholodenko, BN & Hlavacek, WS 2019, 'Modeling cell line-specific recruitment of signaling proteins to the insulin-like growth factor 1 receptor', PLoS Computational Biology, vol. 15, no. 1, pp. e1006706. https://doi.org/10.1371/journal.pcbi.1006706
Erickson, Keesha E. ; Rukhlenko, Oleksii S. ; Shahinuzzaman, Md ; Slavkova, Kalina P. ; Lin, Yen Ting ; Suderman, Ryan ; Stites, Edward C. ; Anghel, Marian ; Posner, Richard G ; Barua, Dipak ; Kholodenko, Boris N. ; Hlavacek, William S. / Modeling cell line-specific recruitment of signaling proteins to the insulin-like growth factor 1 receptor. In: PLoS Computational Biology. 2019 ; Vol. 15, No. 1. pp. e1006706.
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