Mobile elements and the genesis of microsatellites in Dipterans

Jason A Wilder, Hope Hollocher

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Factors that influence the genesis and genomic distribution of microsatellite DNA are poorly understood. We have identified a novel class of Dipteran mobile elements, mini-me elements, which help elucidate both of these issues. These retroposons contain two internal proto-microsatellite regions that commonly expand into lengthy microsatellite repeats. These elements are highly abundant, accounting for approximately 1.2% of the Drosophila melanogaster genome, giving them the potential to be a prolific source of microsatellite DNA variation. They also give us the opportunity to observe the outcomes of multiple microsatellite genesis events (initiating from the same proto-microsatellite) at separate mini-me loci. Based on these observations, we determined that the genesis of microsatellites within mini-me elements occurs through two separate mutational processes: the expansion of preexisting tandem repeats and the conversion of sequence with high cryptic simplicity into tandemly repetitive DNA. These modes of microsatellite genesis can be generalized beyond the case of mini-me elements and help to explain the genesis of microsatellites in any sequence region that is not constrained by selection.

Original languageEnglish (US)
Pages (from-to)384-392
Number of pages9
JournalMolecular Biology and Evolution
Volume18
Issue number3
StatePublished - 2001
Externally publishedYes

Fingerprint

Microsatellite Repeats
microsatellite repeats
DNA
genomics
genome
dipteran
Retroelements
Tandem Repeat Sequences
tandem repeat sequences
retrotransposons
Drosophila melanogaster
Genes
Genome
loci
distribution

Keywords

  • Diptera
  • Drosophila
  • Microsatellite genesis
  • Mobile elements
  • Retroposons

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

Cite this

Mobile elements and the genesis of microsatellites in Dipterans. / Wilder, Jason A; Hollocher, Hope.

In: Molecular Biology and Evolution, Vol. 18, No. 3, 2001, p. 384-392.

Research output: Contribution to journalArticle

@article{efb694d324414afebb9af7cf2ee3d072,
title = "Mobile elements and the genesis of microsatellites in Dipterans",
abstract = "Factors that influence the genesis and genomic distribution of microsatellite DNA are poorly understood. We have identified a novel class of Dipteran mobile elements, mini-me elements, which help elucidate both of these issues. These retroposons contain two internal proto-microsatellite regions that commonly expand into lengthy microsatellite repeats. These elements are highly abundant, accounting for approximately 1.2{\%} of the Drosophila melanogaster genome, giving them the potential to be a prolific source of microsatellite DNA variation. They also give us the opportunity to observe the outcomes of multiple microsatellite genesis events (initiating from the same proto-microsatellite) at separate mini-me loci. Based on these observations, we determined that the genesis of microsatellites within mini-me elements occurs through two separate mutational processes: the expansion of preexisting tandem repeats and the conversion of sequence with high cryptic simplicity into tandemly repetitive DNA. These modes of microsatellite genesis can be generalized beyond the case of mini-me elements and help to explain the genesis of microsatellites in any sequence region that is not constrained by selection.",
keywords = "Diptera, Drosophila, Microsatellite genesis, Mobile elements, Retroposons",
author = "Wilder, {Jason A} and Hope Hollocher",
year = "2001",
language = "English (US)",
volume = "18",
pages = "384--392",
journal = "Molecular Biology and Evolution",
issn = "0737-4038",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - Mobile elements and the genesis of microsatellites in Dipterans

AU - Wilder, Jason A

AU - Hollocher, Hope

PY - 2001

Y1 - 2001

N2 - Factors that influence the genesis and genomic distribution of microsatellite DNA are poorly understood. We have identified a novel class of Dipteran mobile elements, mini-me elements, which help elucidate both of these issues. These retroposons contain two internal proto-microsatellite regions that commonly expand into lengthy microsatellite repeats. These elements are highly abundant, accounting for approximately 1.2% of the Drosophila melanogaster genome, giving them the potential to be a prolific source of microsatellite DNA variation. They also give us the opportunity to observe the outcomes of multiple microsatellite genesis events (initiating from the same proto-microsatellite) at separate mini-me loci. Based on these observations, we determined that the genesis of microsatellites within mini-me elements occurs through two separate mutational processes: the expansion of preexisting tandem repeats and the conversion of sequence with high cryptic simplicity into tandemly repetitive DNA. These modes of microsatellite genesis can be generalized beyond the case of mini-me elements and help to explain the genesis of microsatellites in any sequence region that is not constrained by selection.

AB - Factors that influence the genesis and genomic distribution of microsatellite DNA are poorly understood. We have identified a novel class of Dipteran mobile elements, mini-me elements, which help elucidate both of these issues. These retroposons contain two internal proto-microsatellite regions that commonly expand into lengthy microsatellite repeats. These elements are highly abundant, accounting for approximately 1.2% of the Drosophila melanogaster genome, giving them the potential to be a prolific source of microsatellite DNA variation. They also give us the opportunity to observe the outcomes of multiple microsatellite genesis events (initiating from the same proto-microsatellite) at separate mini-me loci. Based on these observations, we determined that the genesis of microsatellites within mini-me elements occurs through two separate mutational processes: the expansion of preexisting tandem repeats and the conversion of sequence with high cryptic simplicity into tandemly repetitive DNA. These modes of microsatellite genesis can be generalized beyond the case of mini-me elements and help to explain the genesis of microsatellites in any sequence region that is not constrained by selection.

KW - Diptera

KW - Drosophila

KW - Microsatellite genesis

KW - Mobile elements

KW - Retroposons

UR - http://www.scopus.com/inward/record.url?scp=0035100172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035100172&partnerID=8YFLogxK

M3 - Article

C2 - 11230539

AN - SCOPUS:0035100172

VL - 18

SP - 384

EP - 392

JO - Molecular Biology and Evolution

JF - Molecular Biology and Evolution

SN - 0737-4038

IS - 3

ER -