Micropatterned avidin arrays on silicon substrates via photolithography, self-assembly and bioconjugation

Timothy L. Vail, Kevin W. Cushing, Jani C Ingram, Ingrid St. Omer

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Processes for micropatterning protein arrays on inorganic substrates have gained attention in the development of biosensors and clinical diagnostics. This study demonstrates a chemically selective method based on photolithographic deposition of gold patterns with the subsequent attachment of functionalized alkanethiols via molecular self-assembly. Selective capping of carboxy groups on alkanethiols by N-hydroxysuccinimide esters allowed the deposition of methoxypoly(ethylene glycol) silane as a blocking agent on unpatterned regions. Carboxylates were used to couple a form of avidin to create a microarray of protein. This microarray was successfully probed with biotinylated quantum dots. In-process characterization methods included grazing-angle Fourier-transform IR spectroscopy, ellipsometry, contact-angle goniometry, atomic-force microscopy and fluorescence microscopy.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalBiotechnology and Applied Biochemistry
Volume43
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Protein Array Analysis
Avidin
Photolithography
Silicon
Microarrays
Self assembly
Proteins
Silanes
Quantum Dots
Ethylene Glycol
Atomic Force Microscopy
Fluorescence microscopy
Ellipsometry
Biosensing Techniques
Fourier Analysis
Substrates
Ethylene glycol
Fluorescence Microscopy
Biosensors
Gold

Keywords

  • Alkanethiol
  • Avidin
  • Biotin
  • Microarray
  • Quantum dots
  • Self-assembly

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Micropatterned avidin arrays on silicon substrates via photolithography, self-assembly and bioconjugation. / Vail, Timothy L.; Cushing, Kevin W.; Ingram, Jani C; St. Omer, Ingrid.

In: Biotechnology and Applied Biochemistry, Vol. 43, No. 2, 02.2006, p. 85-91.

Research output: Contribution to journalArticle

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