Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms

Research output: Chapter in Book/Report/Conference proceedingChapter

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Abstract

A crude soluble extract (AWH) obtained from homogenized adult Nematospiroides dubius worms was fractionated into normal (NMIgAg) and immune (IMIgAg) antigens by sequential passage through Sepharose 4B, and CNBr-activated Sepharose 4B to which immunoglobulins from normal (NMIg) and N. dubius-infected mouse serum (IMIg) had been coupled. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions showed that MNIgAg and IMIgAg contained several proteins with molecular weights (MW) between 20,000 and 200,000. IMIgAg contained 2 polypeptides, MW 24,000 and 55,000, which reacted strongly in western blot analysis against IMIg; the larger molecule related to a cuticular component of the worm. Protective high MW AWH G-200 filtrates shared 5 polypeptides with IMIgAg (MW 85,000-200,000) together with several smaller molecules (MW 30,000- <10,000). The MW 55,000 and 24,000 components concentrated in IMIgAg were restricted to non-protective low MW AWH gel filtrates. BCF1 mice vaccinated with IMIgAg were protected against N. dubius. Worm numbers, size and fecundity were reduced to an extent commensurate with that obtained in mice vaccinated with AWH. Mice vaccinated with NMIgAg showed partial resistance. The role of surface and dominant parasite immunogens in immunity is discussed.

Original languageEnglish (US)
Title of host publicationImmunology and Cell Biology
Pages223-230
Number of pages8
Volume65
Edition3
StatePublished - 1987
Externally publishedYes

Fingerprint

Nematospiroides dubius
Affinity chromatography
Affinity Chromatography
Molecular Weight
Molecular weight
Antigens
Sepharose
Peptides
Molecules
Electrophoresis
Complex Mixtures
Sodium Dodecyl Sulfate
Fertility
Immunoglobulins
Polyacrylamide Gel Electrophoresis
Immunity
Parasites
Western Blotting
Gels

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Immunology

Cite this

Monroy, F. P., & Dobson, C. (1987). Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms. In Immunology and Cell Biology (3 ed., Vol. 65, pp. 223-230)

Mice vaccinated against Nematospiroides dubius with antigens isolated by affinity chromatography from adult worms. / Monroy, Fernando P; Dobson, C.

Immunology and Cell Biology. Vol. 65 3. ed. 1987. p. 223-230.

Research output: Chapter in Book/Report/Conference proceedingChapter

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