Metal transformation by a novel Pelosinus isolate from a subsurface environment

Allison E. Ray, Stephanie A. Connon, Andrew L. Neal, Yoshiko Fujita, David E. Cummings, Jani C. Ingram, Timothy S. Magnuson

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. A metal-reducing microbe, "Pelosinus lilae" strain UFO1, was isolated under strictly anaerobic conditions from an Fe(III)-reducing enrichment established with uncontaminated soil from the Department of Energy Oak Ridge Field Research Center, Tennessee. "P. lilae" UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate as carbon and electron donor. Ferrihydrite was not reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 h in suspensions containing 108 cells mL-1 when provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 h. Strain UFO1 is a novel species within the bacterial genus Pelosinus, having 98.16% 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol%, and DNA-DNA hybridization of "P. lilae" UFO1 against P. fermentans R7T indicated an average 16.8% DNA-DNA similarity. The unique phylogenetic, physiologic, and metal-transforming characteristics of "P. lilae" UFO1 reveal it is a novel isolate of the described genus Pelosinus.

Original languageEnglish (US)
Article number1689
JournalFrontiers in Microbiology
Volume9
Issue numberAUG
DOIs
StatePublished - Aug 17 2018

Keywords

  • Bioremediation
  • Fermentative bacterium
  • Pelosinus
  • Subsurface environment
  • Toxic metal reduction

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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    Ray, A. E., Connon, S. A., Neal, A. L., Fujita, Y., Cummings, D. E., Ingram, J. C., & Magnuson, T. S. (2018). Metal transformation by a novel Pelosinus isolate from a subsurface environment. Frontiers in Microbiology, 9(AUG), [1689]. https://doi.org/10.3389/fmicb.2018.01689