Metal transformation by a novel Pelosinus isolate from a subsurface environment

Allison E. Ray, Stephanie A. Connon, Andrew L. Neal, Yoshiko Fujita, David E. Cummings, Jani C Ingram, Timothy S. Magnuson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. A metal-reducing microbe, "Pelosinus lilae" strain UFO1, was isolated under strictly anaerobic conditions from an Fe(III)-reducing enrichment established with uncontaminated soil from the Department of Energy Oak Ridge Field Research Center, Tennessee. "P. lilae" UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate as carbon and electron donor. Ferrihydrite was not reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 h in suspensions containing 108 cells mL-1 when provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 h. Strain UFO1 is a novel species within the bacterial genus Pelosinus, having 98.16% 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol%, and DNA-DNA hybridization of "P. lilae" UFO1 against P. fermentans R7T indicated an average 16.8% DNA-DNA similarity. The unique phylogenetic, physiologic, and metal-transforming characteristics of "P. lilae" UFO1 reveal it is a novel isolate of the described genus Pelosinus.

Original languageEnglish (US)
Article number1689
JournalFrontiers in Microbiology
Volume9
Issue numberAUG
DOIs
StatePublished - Aug 17 2018

Fingerprint

Metals
DNA
Lactic Acid
Nitrilotriacetic Acid
Humic Substances
Poisons
Base Composition
Spores
rRNA Genes
Immobilization
Suspensions
Soil
Carbon
Electrons
Research
anthraquinone-2,6-disulfonate
chromium hexavalent ion

Keywords

  • Bioremediation
  • Fermentative bacterium
  • Pelosinus
  • Subsurface environment
  • Toxic metal reduction

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Ray, A. E., Connon, S. A., Neal, A. L., Fujita, Y., Cummings, D. E., Ingram, J. C., & Magnuson, T. S. (2018). Metal transformation by a novel Pelosinus isolate from a subsurface environment. Frontiers in Microbiology, 9(AUG), [1689]. https://doi.org/10.3389/fmicb.2018.01689

Metal transformation by a novel Pelosinus isolate from a subsurface environment. / Ray, Allison E.; Connon, Stephanie A.; Neal, Andrew L.; Fujita, Yoshiko; Cummings, David E.; Ingram, Jani C; Magnuson, Timothy S.

In: Frontiers in Microbiology, Vol. 9, No. AUG, 1689, 17.08.2018.

Research output: Contribution to journalArticle

Ray, AE, Connon, SA, Neal, AL, Fujita, Y, Cummings, DE, Ingram, JC & Magnuson, TS 2018, 'Metal transformation by a novel Pelosinus isolate from a subsurface environment', Frontiers in Microbiology, vol. 9, no. AUG, 1689. https://doi.org/10.3389/fmicb.2018.01689
Ray, Allison E. ; Connon, Stephanie A. ; Neal, Andrew L. ; Fujita, Yoshiko ; Cummings, David E. ; Ingram, Jani C ; Magnuson, Timothy S. / Metal transformation by a novel Pelosinus isolate from a subsurface environment. In: Frontiers in Microbiology. 2018 ; Vol. 9, No. AUG.
@article{2b56278d48f24e70903e4b8f5cef01b3,
title = "Metal transformation by a novel Pelosinus isolate from a subsurface environment",
abstract = "The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. A metal-reducing microbe, {"}Pelosinus lilae{"} strain UFO1, was isolated under strictly anaerobic conditions from an Fe(III)-reducing enrichment established with uncontaminated soil from the Department of Energy Oak Ridge Field Research Center, Tennessee. {"}P. lilae{"} UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate as carbon and electron donor. Ferrihydrite was not reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 h in suspensions containing 108 cells mL-1 when provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 h. Strain UFO1 is a novel species within the bacterial genus Pelosinus, having 98.16{\%} 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol{\%}, and DNA-DNA hybridization of {"}P. lilae{"} UFO1 against P. fermentans R7T indicated an average 16.8{\%} DNA-DNA similarity. The unique phylogenetic, physiologic, and metal-transforming characteristics of {"}P. lilae{"} UFO1 reveal it is a novel isolate of the described genus Pelosinus.",
keywords = "Bioremediation, Fermentative bacterium, Pelosinus, Subsurface environment, Toxic metal reduction",
author = "Ray, {Allison E.} and Connon, {Stephanie A.} and Neal, {Andrew L.} and Yoshiko Fujita and Cummings, {David E.} and Ingram, {Jani C} and Magnuson, {Timothy S.}",
year = "2018",
month = "8",
day = "17",
doi = "10.3389/fmicb.2018.01689",
language = "English (US)",
volume = "9",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
number = "AUG",

}

TY - JOUR

T1 - Metal transformation by a novel Pelosinus isolate from a subsurface environment

AU - Ray, Allison E.

AU - Connon, Stephanie A.

AU - Neal, Andrew L.

AU - Fujita, Yoshiko

AU - Cummings, David E.

AU - Ingram, Jani C

AU - Magnuson, Timothy S.

PY - 2018/8/17

Y1 - 2018/8/17

N2 - The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. A metal-reducing microbe, "Pelosinus lilae" strain UFO1, was isolated under strictly anaerobic conditions from an Fe(III)-reducing enrichment established with uncontaminated soil from the Department of Energy Oak Ridge Field Research Center, Tennessee. "P. lilae" UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate as carbon and electron donor. Ferrihydrite was not reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 h in suspensions containing 108 cells mL-1 when provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 h. Strain UFO1 is a novel species within the bacterial genus Pelosinus, having 98.16% 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol%, and DNA-DNA hybridization of "P. lilae" UFO1 against P. fermentans R7T indicated an average 16.8% DNA-DNA similarity. The unique phylogenetic, physiologic, and metal-transforming characteristics of "P. lilae" UFO1 reveal it is a novel isolate of the described genus Pelosinus.

AB - The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. A metal-reducing microbe, "Pelosinus lilae" strain UFO1, was isolated under strictly anaerobic conditions from an Fe(III)-reducing enrichment established with uncontaminated soil from the Department of Energy Oak Ridge Field Research Center, Tennessee. "P. lilae" UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate as carbon and electron donor. Ferrihydrite was not reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 h in suspensions containing 108 cells mL-1 when provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 h. Strain UFO1 is a novel species within the bacterial genus Pelosinus, having 98.16% 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol%, and DNA-DNA hybridization of "P. lilae" UFO1 against P. fermentans R7T indicated an average 16.8% DNA-DNA similarity. The unique phylogenetic, physiologic, and metal-transforming characteristics of "P. lilae" UFO1 reveal it is a novel isolate of the described genus Pelosinus.

KW - Bioremediation

KW - Fermentative bacterium

KW - Pelosinus

KW - Subsurface environment

KW - Toxic metal reduction

UR - http://www.scopus.com/inward/record.url?scp=85051643331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051643331&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2018.01689

DO - 10.3389/fmicb.2018.01689

M3 - Article

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - AUG

M1 - 1689

ER -