Medical therapy reduces microbiota diversity and evenness in surgically recalcitrant chronic rhinosinusitis

Cindy M. Liu, Katerina Soldanova, Lora Nordstrom, Michael G. Dwan, Owain L. Moss, Tania L. Contente-Cuomo, Paul S Keim, Lance B. Price, Andrew P. Lane

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Background: Chronic rhinosinusitis (CRS) is a highly prevalent and heterogeneous condition frequently treated with antibiotics and corticosteroid therapy. However, the effect of medical therapy on sinus microbiota remains unknown. Methods: We enrolled CRS patients (n = 6) with patent maxillary antrostomies and active mucosal inflammation, who had not received antibiotics or corticosteroids in the previous 8 weeks. A pretreatment and posttreatment maxillary sinus swab was collected, from which DNA was extracted, pyrosequenced, and analyzed using a naïve Bayesian classifier and ecological analyses. Results: Four patients showed significant improvement in endoscopic appearance. The shifts in microbiota in response to therapy were highly individualized. There was no single common microbiota profile among patients with similar clinical outcomes, but overall there was significant decrease in microbiota diversity (t(5) = 2.05, p = 0.10) and evenness (t(5) = 2.28, p = 0.07) after treatment. Conclusion: Our findings strongly correlate with earlier studies that examined the impact of antibiotics on human microbiota. We observed that posttreatment, patients frequently became colonized by taxa that are less susceptible to the prescribed antibiotics. Our findings highlight the challenge in seeking generalizable diagnostic and therapeutic options in CRS, particularly regarding microbiological response and outcomes.

Original languageEnglish (US)
Pages (from-to)775-781
Number of pages7
JournalInternational Forum of Allergy and Rhinology
Volume3
Issue number10
DOIs
StatePublished - Oct 2013

Fingerprint

Microbiota
Anti-Bacterial Agents
Adrenal Cortex Hormones
Therapeutics
Maxillary Sinus
Inflammation
DNA

Keywords

  • 16S rRNA gene
  • Chronic rhinosinusitis
  • Maximum medical therapy
  • Microbiology
  • Microbiome
  • Microbiota
  • Nasal polyposis
  • Sinusitis, eosinophilic rhinitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology

Cite this

Liu, C. M., Soldanova, K., Nordstrom, L., Dwan, M. G., Moss, O. L., Contente-Cuomo, T. L., ... Lane, A. P. (2013). Medical therapy reduces microbiota diversity and evenness in surgically recalcitrant chronic rhinosinusitis. International Forum of Allergy and Rhinology, 3(10), 775-781. https://doi.org/10.1002/alr.21195

Medical therapy reduces microbiota diversity and evenness in surgically recalcitrant chronic rhinosinusitis. / Liu, Cindy M.; Soldanova, Katerina; Nordstrom, Lora; Dwan, Michael G.; Moss, Owain L.; Contente-Cuomo, Tania L.; Keim, Paul S; Price, Lance B.; Lane, Andrew P.

In: International Forum of Allergy and Rhinology, Vol. 3, No. 10, 10.2013, p. 775-781.

Research output: Contribution to journalArticle

Liu, CM, Soldanova, K, Nordstrom, L, Dwan, MG, Moss, OL, Contente-Cuomo, TL, Keim, PS, Price, LB & Lane, AP 2013, 'Medical therapy reduces microbiota diversity and evenness in surgically recalcitrant chronic rhinosinusitis', International Forum of Allergy and Rhinology, vol. 3, no. 10, pp. 775-781. https://doi.org/10.1002/alr.21195
Liu, Cindy M. ; Soldanova, Katerina ; Nordstrom, Lora ; Dwan, Michael G. ; Moss, Owain L. ; Contente-Cuomo, Tania L. ; Keim, Paul S ; Price, Lance B. ; Lane, Andrew P. / Medical therapy reduces microbiota diversity and evenness in surgically recalcitrant chronic rhinosinusitis. In: International Forum of Allergy and Rhinology. 2013 ; Vol. 3, No. 10. pp. 775-781.
@article{e529598c9c5c475aae2d44c994959d56,
title = "Medical therapy reduces microbiota diversity and evenness in surgically recalcitrant chronic rhinosinusitis",
abstract = "Background: Chronic rhinosinusitis (CRS) is a highly prevalent and heterogeneous condition frequently treated with antibiotics and corticosteroid therapy. However, the effect of medical therapy on sinus microbiota remains unknown. Methods: We enrolled CRS patients (n = 6) with patent maxillary antrostomies and active mucosal inflammation, who had not received antibiotics or corticosteroids in the previous 8 weeks. A pretreatment and posttreatment maxillary sinus swab was collected, from which DNA was extracted, pyrosequenced, and analyzed using a na{\"i}ve Bayesian classifier and ecological analyses. Results: Four patients showed significant improvement in endoscopic appearance. The shifts in microbiota in response to therapy were highly individualized. There was no single common microbiota profile among patients with similar clinical outcomes, but overall there was significant decrease in microbiota diversity (t(5) = 2.05, p = 0.10) and evenness (t(5) = 2.28, p = 0.07) after treatment. Conclusion: Our findings strongly correlate with earlier studies that examined the impact of antibiotics on human microbiota. We observed that posttreatment, patients frequently became colonized by taxa that are less susceptible to the prescribed antibiotics. Our findings highlight the challenge in seeking generalizable diagnostic and therapeutic options in CRS, particularly regarding microbiological response and outcomes.",
keywords = "16S rRNA gene, Chronic rhinosinusitis, Maximum medical therapy, Microbiology, Microbiome, Microbiota, Nasal polyposis, Sinusitis, eosinophilic rhinitis",
author = "Liu, {Cindy M.} and Katerina Soldanova and Lora Nordstrom and Dwan, {Michael G.} and Moss, {Owain L.} and Contente-Cuomo, {Tania L.} and Keim, {Paul S} and Price, {Lance B.} and Lane, {Andrew P.}",
year = "2013",
month = "10",
doi = "10.1002/alr.21195",
language = "English (US)",
volume = "3",
pages = "775--781",
journal = "International Forum of Allergy and Rhinology",
issn = "2042-6976",
publisher = "Wiley-Blackwell",
number = "10",

}

TY - JOUR

T1 - Medical therapy reduces microbiota diversity and evenness in surgically recalcitrant chronic rhinosinusitis

AU - Liu, Cindy M.

AU - Soldanova, Katerina

AU - Nordstrom, Lora

AU - Dwan, Michael G.

AU - Moss, Owain L.

AU - Contente-Cuomo, Tania L.

AU - Keim, Paul S

AU - Price, Lance B.

AU - Lane, Andrew P.

PY - 2013/10

Y1 - 2013/10

N2 - Background: Chronic rhinosinusitis (CRS) is a highly prevalent and heterogeneous condition frequently treated with antibiotics and corticosteroid therapy. However, the effect of medical therapy on sinus microbiota remains unknown. Methods: We enrolled CRS patients (n = 6) with patent maxillary antrostomies and active mucosal inflammation, who had not received antibiotics or corticosteroids in the previous 8 weeks. A pretreatment and posttreatment maxillary sinus swab was collected, from which DNA was extracted, pyrosequenced, and analyzed using a naïve Bayesian classifier and ecological analyses. Results: Four patients showed significant improvement in endoscopic appearance. The shifts in microbiota in response to therapy were highly individualized. There was no single common microbiota profile among patients with similar clinical outcomes, but overall there was significant decrease in microbiota diversity (t(5) = 2.05, p = 0.10) and evenness (t(5) = 2.28, p = 0.07) after treatment. Conclusion: Our findings strongly correlate with earlier studies that examined the impact of antibiotics on human microbiota. We observed that posttreatment, patients frequently became colonized by taxa that are less susceptible to the prescribed antibiotics. Our findings highlight the challenge in seeking generalizable diagnostic and therapeutic options in CRS, particularly regarding microbiological response and outcomes.

AB - Background: Chronic rhinosinusitis (CRS) is a highly prevalent and heterogeneous condition frequently treated with antibiotics and corticosteroid therapy. However, the effect of medical therapy on sinus microbiota remains unknown. Methods: We enrolled CRS patients (n = 6) with patent maxillary antrostomies and active mucosal inflammation, who had not received antibiotics or corticosteroids in the previous 8 weeks. A pretreatment and posttreatment maxillary sinus swab was collected, from which DNA was extracted, pyrosequenced, and analyzed using a naïve Bayesian classifier and ecological analyses. Results: Four patients showed significant improvement in endoscopic appearance. The shifts in microbiota in response to therapy were highly individualized. There was no single common microbiota profile among patients with similar clinical outcomes, but overall there was significant decrease in microbiota diversity (t(5) = 2.05, p = 0.10) and evenness (t(5) = 2.28, p = 0.07) after treatment. Conclusion: Our findings strongly correlate with earlier studies that examined the impact of antibiotics on human microbiota. We observed that posttreatment, patients frequently became colonized by taxa that are less susceptible to the prescribed antibiotics. Our findings highlight the challenge in seeking generalizable diagnostic and therapeutic options in CRS, particularly regarding microbiological response and outcomes.

KW - 16S rRNA gene

KW - Chronic rhinosinusitis

KW - Maximum medical therapy

KW - Microbiology

KW - Microbiome

KW - Microbiota

KW - Nasal polyposis

KW - Sinusitis, eosinophilic rhinitis

UR - http://www.scopus.com/inward/record.url?scp=84885868981&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885868981&partnerID=8YFLogxK

U2 - 10.1002/alr.21195

DO - 10.1002/alr.21195

M3 - Article

C2 - 23843343

AN - SCOPUS:84885868981

VL - 3

SP - 775

EP - 781

JO - International Forum of Allergy and Rhinology

JF - International Forum of Allergy and Rhinology

SN - 2042-6976

IS - 10

ER -