Measuring nitrification, denitrification, and related biomarkers in terrestrial geothermal ecosystems

Jeremy A. Dodsworth, Bruce A Hungate, José R. De La Torre, Hongchen Jiang, Brian P. Hedlund

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Research on the nitrogen biogeochemical cycle in terrestrial geothermal ecosystems has recently been energized by the discovery of thermophilic ammonia-oxidizing archaea (AOA). This chapter describes methods that have been used for measuring nitrification and denitrification in hot spring environments, including isotope pool dilution and tracer approaches, and the acetylene block approach. The chapter also summarizes qualitative and quantitative methods for measurement of functional and phylogenetic biomarkers of thermophiles potentially involved in these processes.

Original languageEnglish (US)
Pages (from-to)171-203
Number of pages33
JournalMethods in Enzymology
Volume486
Issue numberC
DOIs
StatePublished - 2010

Fingerprint

Hot springs
Nitrification
Denitrification
Acetylene
Biomarkers
Ammonia
Isotopes
Ecosystems
Dilution
Ecosystem
Nitrogen
Nitrogen Cycle
Hot Springs
Archaea
Research

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Measuring nitrification, denitrification, and related biomarkers in terrestrial geothermal ecosystems. / Dodsworth, Jeremy A.; Hungate, Bruce A; De La Torre, José R.; Jiang, Hongchen; Hedlund, Brian P.

In: Methods in Enzymology, Vol. 486, No. C, 2010, p. 171-203.

Research output: Contribution to journalArticle

Dodsworth, Jeremy A. ; Hungate, Bruce A ; De La Torre, José R. ; Jiang, Hongchen ; Hedlund, Brian P. / Measuring nitrification, denitrification, and related biomarkers in terrestrial geothermal ecosystems. In: Methods in Enzymology. 2010 ; Vol. 486, No. C. pp. 171-203.
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