Measuring nitrification, denitrification, and related biomarkers in terrestrial geothermal ecosystems

Jeremy A. Dodsworth, Bruce Hungate, José R. De La Torre, Hongchen Jiang, Brian P. Hedlund

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Research on the nitrogen biogeochemical cycle in terrestrial geothermal ecosystems has recently been energized by the discovery of thermophilic ammonia-oxidizing archaea (AOA). This chapter describes methods that have been used for measuring nitrification and denitrification in hot spring environments, including isotope pool dilution and tracer approaches, and the acetylene block approach. The chapter also summarizes qualitative and quantitative methods for measurement of functional and phylogenetic biomarkers of thermophiles potentially involved in these processes.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages171-203
Number of pages33
EditionC
DOIs
StatePublished - Jan 1 2011

Publication series

NameMethods in Enzymology
NumberC
Volume486
ISSN (Print)0076-6879

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Dodsworth, J. A., Hungate, B., De La Torre, J. R., Jiang, H., & Hedlund, B. P. (2011). Measuring nitrification, denitrification, and related biomarkers in terrestrial geothermal ecosystems. In Methods in Enzymology (C ed., pp. 171-203). (Methods in Enzymology; Vol. 486, No. C). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-381294-0.00008-0