Abstract
Research on the nitrogen biogeochemical cycle in terrestrial geothermal ecosystems has recently been energized by the discovery of thermophilic ammonia-oxidizing archaea (AOA). This chapter describes methods that have been used for measuring nitrification and denitrification in hot spring environments, including isotope pool dilution and tracer approaches, and the acetylene block approach. The chapter also summarizes qualitative and quantitative methods for measurement of functional and phylogenetic biomarkers of thermophiles potentially involved in these processes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 171-203 |
| Number of pages | 33 |
| Journal | Methods in Enzymology |
| Volume | 486 |
| Issue number | C |
| DOIs | |
| State | Published - 2010 |
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ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
Measuring nitrification, denitrification, and related biomarkers in terrestrial geothermal ecosystems. / Dodsworth, Jeremy A.; Hungate, Bruce A; De La Torre, José R.; Jiang, Hongchen; Hedlund, Brian P.
In: Methods in Enzymology, Vol. 486, No. C, 2010, p. 171-203.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measuring nitrification, denitrification, and related biomarkers in terrestrial geothermal ecosystems
AU - Dodsworth, Jeremy A.
AU - Hungate, Bruce A
AU - De La Torre, José R.
AU - Jiang, Hongchen
AU - Hedlund, Brian P.
PY - 2010
Y1 - 2010
N2 - Research on the nitrogen biogeochemical cycle in terrestrial geothermal ecosystems has recently been energized by the discovery of thermophilic ammonia-oxidizing archaea (AOA). This chapter describes methods that have been used for measuring nitrification and denitrification in hot spring environments, including isotope pool dilution and tracer approaches, and the acetylene block approach. The chapter also summarizes qualitative and quantitative methods for measurement of functional and phylogenetic biomarkers of thermophiles potentially involved in these processes.
AB - Research on the nitrogen biogeochemical cycle in terrestrial geothermal ecosystems has recently been energized by the discovery of thermophilic ammonia-oxidizing archaea (AOA). This chapter describes methods that have been used for measuring nitrification and denitrification in hot spring environments, including isotope pool dilution and tracer approaches, and the acetylene block approach. The chapter also summarizes qualitative and quantitative methods for measurement of functional and phylogenetic biomarkers of thermophiles potentially involved in these processes.
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UR - http://www.scopus.com/inward/citedby.url?scp=78650956795&partnerID=8YFLogxK
U2 - 10.1016/B978-0-12-381294-0.00008-0
DO - 10.1016/B978-0-12-381294-0.00008-0
M3 - Article
VL - 486
SP - 171
EP - 203
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -