Measuring growth of a phenanthrene-degrading bacterial inoculum in soil with a quantitative competitive polymerase chain reaction method

Egbert Schwartz, Sinh V. Trinh, Kate M. Scow

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We measured growth of a phenanthrene-degrading bacterium, Arthrobacter, strain RP17, in Forbes soil, amended with 500 μg g-1 phenanthrene using a quantitative competitive polymerase chain reaction method. The inoculum, which was not indigenous to Forbes soil, grew from 5.55x105 colony forming units (cfu) g-1 to 1.97x107 cfu g-1 within 100 h after the cells were added to the soil. Maximum population density was reached before the highest degradation rate was observed 150 h after the cells were added to soil. Population density remained stable even after 56% of the phenanthrene had mineralized. This study is one of the few documented examples of growth by a non-indigenous bacterium in a non-sterile soil amended with a pollutant. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalFEMS Microbiology Ecology
Volume34
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

phenanthrene
polymerase chain reaction
Soil
Polymerase Chain Reaction
Growth
Population Density
soil
population density
Stem Cells
Arthrobacter
Bacteria
bacterium
measuring
method
degradation
pollutant

Keywords

  • Bioaugmentation
  • Bioremediation
  • Phenanthrene degradation
  • Quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Measuring growth of a phenanthrene-degrading bacterial inoculum in soil with a quantitative competitive polymerase chain reaction method. / Schwartz, Egbert; Trinh, Sinh V.; Scow, Kate M.

In: FEMS Microbiology Ecology, Vol. 34, No. 1, 2000, p. 1-7.

Research output: Contribution to journalArticle

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