Mapping and comparing bacterial microbiota in the sinonasal cavity of healthy, allergic rhinitis, and chronic rhinosinusitis subjects

Devyani Lal, Paul S Keim, Josie Delisle, Bridget Barker, Matthew A. Rank, Nicholas Chia, James M. Schupp, John D. Gillece, Emily K. Cope

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: The role of microbiota in sinonasal inflammation can be further understood by targeted sampling of healthy and diseased subjects. We compared the microbiota of the middle meatus (MM) and inferior meatus (IM) in healthy, allergic rhinitis (AR), and chronic rhinosinusitis (CRS) subjects to characterize intrasubject, intersubject, and intergroup differences. Methods: Subjects were recruited in the office, and characterized into healthy, AR, and CRS groups. Endoscopically-guided swab samples were obtained from the MM and IM bilaterally. Bacterial microbiota were characterized by sequencing the V3-V4 region of the 16S ribosomal RNA (rRNA) gene. Results: Intersubject microbiome analyses were conducted in 65 subjects: 8 healthy, 11 AR, and 46 CRS (25 CRS with nasal polyps [CRSwNP]; 21 CRS without nasal polyps [CRSsNP]). Intrasubject analyses were conducted for 48 individuals (4 controls, 11 AR, 8 CRSwNP, and 15 CRSwNP). There was considerable intersubject microbiota variability, but intrasubject profiles were similar (p = 0.001, nonparametric t test). Intrasubject bacterial diversity was significantly reduced in MM of CRSsNP subjects compared to IM samples (p = 0.022, nonparametric t test). CRSsNP MM samples were enriched in Streptococcus, Haemophilus, and Fusobacterium spp. but exhibited loss of diversity compared to healthy, CRSwNP, and AR subject-samples (p < 0.05; nonparametric t test). CRSwNP patients were enriched in Staphylococcus, Alloiococcus, and Corynebacterium spp. Conclusion: This study presents the sinonasal microbiome profile in one of the larger populations of non-CRS and CRS subjects, and is the first office-based cohort in the literature. In contrast to healthy, AR, and CRSwNP subjects, CRSsNP MM samples exhibited decreased microbiome diversity and anaerobic enrichment. CRSsNP MM samples had reduced diversity compared to same-subject IM samples, a novel finding.

Original languageEnglish (US)
Pages (from-to)561-569
Number of pages9
JournalInternational Forum of Allergy and Rhinology
Volume7
Issue number6
DOIs
StatePublished - Jun 1 2017

Fingerprint

Nasal Polyps
Microbiota
Healthy Volunteers
Fusobacterium
Haemophilus
Corynebacterium
Allergic Rhinitis
Streptococcus
Staphylococcus
Inflammation
Population
Genes

Keywords

  • allergic rhinitis
  • bacteriology
  • chronic rhinosinusitis
  • inferior meatus
  • microbioata
  • microbiome
  • middle meatus
  • nasal polyposis
  • regional sinonasal microbiota and rhinosinusitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology

Cite this

Mapping and comparing bacterial microbiota in the sinonasal cavity of healthy, allergic rhinitis, and chronic rhinosinusitis subjects. / Lal, Devyani; Keim, Paul S; Delisle, Josie; Barker, Bridget; Rank, Matthew A.; Chia, Nicholas; Schupp, James M.; Gillece, John D.; Cope, Emily K.

In: International Forum of Allergy and Rhinology, Vol. 7, No. 6, 01.06.2017, p. 561-569.

Research output: Contribution to journalArticle

Lal, Devyani ; Keim, Paul S ; Delisle, Josie ; Barker, Bridget ; Rank, Matthew A. ; Chia, Nicholas ; Schupp, James M. ; Gillece, John D. ; Cope, Emily K. / Mapping and comparing bacterial microbiota in the sinonasal cavity of healthy, allergic rhinitis, and chronic rhinosinusitis subjects. In: International Forum of Allergy and Rhinology. 2017 ; Vol. 7, No. 6. pp. 561-569.
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AU - Delisle, Josie

AU - Barker, Bridget

AU - Rank, Matthew A.

AU - Chia, Nicholas

AU - Schupp, James M.

AU - Gillece, John D.

AU - Cope, Emily K.

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N2 - Background: The role of microbiota in sinonasal inflammation can be further understood by targeted sampling of healthy and diseased subjects. We compared the microbiota of the middle meatus (MM) and inferior meatus (IM) in healthy, allergic rhinitis (AR), and chronic rhinosinusitis (CRS) subjects to characterize intrasubject, intersubject, and intergroup differences. Methods: Subjects were recruited in the office, and characterized into healthy, AR, and CRS groups. Endoscopically-guided swab samples were obtained from the MM and IM bilaterally. Bacterial microbiota were characterized by sequencing the V3-V4 region of the 16S ribosomal RNA (rRNA) gene. Results: Intersubject microbiome analyses were conducted in 65 subjects: 8 healthy, 11 AR, and 46 CRS (25 CRS with nasal polyps [CRSwNP]; 21 CRS without nasal polyps [CRSsNP]). Intrasubject analyses were conducted for 48 individuals (4 controls, 11 AR, 8 CRSwNP, and 15 CRSwNP). There was considerable intersubject microbiota variability, but intrasubject profiles were similar (p = 0.001, nonparametric t test). Intrasubject bacterial diversity was significantly reduced in MM of CRSsNP subjects compared to IM samples (p = 0.022, nonparametric t test). CRSsNP MM samples were enriched in Streptococcus, Haemophilus, and Fusobacterium spp. but exhibited loss of diversity compared to healthy, CRSwNP, and AR subject-samples (p < 0.05; nonparametric t test). CRSwNP patients were enriched in Staphylococcus, Alloiococcus, and Corynebacterium spp. Conclusion: This study presents the sinonasal microbiome profile in one of the larger populations of non-CRS and CRS subjects, and is the first office-based cohort in the literature. In contrast to healthy, AR, and CRSwNP subjects, CRSsNP MM samples exhibited decreased microbiome diversity and anaerobic enrichment. CRSsNP MM samples had reduced diversity compared to same-subject IM samples, a novel finding.

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