Limitations of the lignin peroxidase system of the white-rot fungus, Phanerochaete chrysosporium

J. L. Kay-Shoemake, Maribeth E Watwood

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The lignin peroxidase enzyme system of the white-rot fungus, Phanerochaete chrysosporium, was assayed for its capacity to degrade two recalcitrant aliphatic ether compounds, high-molecular-mass polyethylene glycol (PEG 20,000) and methyl tert-butyl ether. Ligninolytic cultures of Phanerochaete chrysosporium were spiked with each ether compound and incubated in reaction vessels. Separate incubations were conducted in which the ether compounds were present as sole carbon source. Other parameters, such as varying the methyl tert-butyl ether concentration and veratryl alcohol additions were tested. No significant degradation of either compound was observed under any of the conditions tested. Implications of these results are discussed with respect to the oxidative limitations of the lignin peroxidase enzyme system and structural features of substrate molecules that may be requisite for oxidation by this system.

Original languageEnglish (US)
Pages (from-to)438-442
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume46
Issue number4
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Phanerochaete
Lignin
Fungi
Ether
Ethers
Polyethylene glycols
Molecular mass
Enzymes
Carbon
Degradation
Oxidation
Molecules
Substrates
Alcohols
lignin peroxidase
methyl tert-butyl ether

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Limitations of the lignin peroxidase system of the white-rot fungus, Phanerochaete chrysosporium. / Kay-Shoemake, J. L.; Watwood, Maribeth E.

In: Applied Microbiology and Biotechnology, Vol. 46, No. 4, 1996, p. 438-442.

Research output: Contribution to journalArticle

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