Abstract
Genomic studies have been used to identify genes underlying many important plant secondary metabolic pathways. However, genes for salicinoid phenolic glycosides (SPGs)-ecologically important compounds with significant commercial, cultural, and medicinal applications-remain largely undescribed. We used a linkage map derived from a full-sib population of hybrid cottonwoods (Populus spp.) to search for quantitative trait loci (QTL) for the SPGs salicortin and HCH-salicortin. SSR markers and primer sequences were used to anchor the map to the V3.0 P. trichocarpa genome. We discovered 21 QTL for the two traits, including a major QTL for HCH-salicortin (R2 = .52) that colocated with a QTL for salicortin on chr12. Using the V3.0 Populus genome sequence, we identified 2,983 annotated genes and 1,480 genes of unknown function within our QTL intervals. We note ten candidate genes of interest, including a BAHD-type acyltransferase that has been potentially linked to PopulusSPGs. Our results complement other recent studies in Populus with implications for gene discovery and the evolution of defensive chemistry in a model genus. To our knowledge, this is the first study to use a full-sib mapping population to identify QTL intervals and gene lists associated with SPGs.
| Original language | English (US) |
|---|---|
| Journal | Ecology and Evolution |
| DOIs | |
| State | Accepted/In press - Jan 1 2018 |
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Keywords
- Community and ecosystem genetics
- Defensive chemistry genes
- Foundation species
- Populus
- Quantitative trait loci mapping
- Salicinoid phenolic glycoside
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Ecology
- Nature and Landscape Conservation
Cite this
Large effect quantitative trait loci for salicinoid phenolic glycosides in Populus : Implications for gene discovery. / Woolbright, Scott A.; Rehill, Brian J.; Lindroth, Richard L.; Difazio, Stephen P.; Martinsen, Gregory D.; Zinkgraf, Matthew S.; Allan, Gerard J; Keim, Paul S; Whitham, Thomas G.
In: Ecology and Evolution, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Large effect quantitative trait loci for salicinoid phenolic glycosides in Populus
T2 - Implications for gene discovery
AU - Woolbright, Scott A.
AU - Rehill, Brian J.
AU - Lindroth, Richard L.
AU - Difazio, Stephen P.
AU - Martinsen, Gregory D.
AU - Zinkgraf, Matthew S.
AU - Allan, Gerard J
AU - Keim, Paul S
AU - Whitham, Thomas G
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Genomic studies have been used to identify genes underlying many important plant secondary metabolic pathways. However, genes for salicinoid phenolic glycosides (SPGs)-ecologically important compounds with significant commercial, cultural, and medicinal applications-remain largely undescribed. We used a linkage map derived from a full-sib population of hybrid cottonwoods (Populus spp.) to search for quantitative trait loci (QTL) for the SPGs salicortin and HCH-salicortin. SSR markers and primer sequences were used to anchor the map to the V3.0 P. trichocarpa genome. We discovered 21 QTL for the two traits, including a major QTL for HCH-salicortin (R2 = .52) that colocated with a QTL for salicortin on chr12. Using the V3.0 Populus genome sequence, we identified 2,983 annotated genes and 1,480 genes of unknown function within our QTL intervals. We note ten candidate genes of interest, including a BAHD-type acyltransferase that has been potentially linked to PopulusSPGs. Our results complement other recent studies in Populus with implications for gene discovery and the evolution of defensive chemistry in a model genus. To our knowledge, this is the first study to use a full-sib mapping population to identify QTL intervals and gene lists associated with SPGs.
AB - Genomic studies have been used to identify genes underlying many important plant secondary metabolic pathways. However, genes for salicinoid phenolic glycosides (SPGs)-ecologically important compounds with significant commercial, cultural, and medicinal applications-remain largely undescribed. We used a linkage map derived from a full-sib population of hybrid cottonwoods (Populus spp.) to search for quantitative trait loci (QTL) for the SPGs salicortin and HCH-salicortin. SSR markers and primer sequences were used to anchor the map to the V3.0 P. trichocarpa genome. We discovered 21 QTL for the two traits, including a major QTL for HCH-salicortin (R2 = .52) that colocated with a QTL for salicortin on chr12. Using the V3.0 Populus genome sequence, we identified 2,983 annotated genes and 1,480 genes of unknown function within our QTL intervals. We note ten candidate genes of interest, including a BAHD-type acyltransferase that has been potentially linked to PopulusSPGs. Our results complement other recent studies in Populus with implications for gene discovery and the evolution of defensive chemistry in a model genus. To our knowledge, this is the first study to use a full-sib mapping population to identify QTL intervals and gene lists associated with SPGs.
KW - Community and ecosystem genetics
KW - Defensive chemistry genes
KW - Foundation species
KW - Populus
KW - Quantitative trait loci mapping
KW - Salicinoid phenolic glycoside
UR - http://www.scopus.com/inward/record.url?scp=85043370857&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85043370857&partnerID=8YFLogxK
U2 - 10.1002/ece3.3932
DO - 10.1002/ece3.3932
M3 - Article
AN - SCOPUS:85043370857
JO - Ecology and Evolution
JF - Ecology and Evolution
SN - 2045-7758
ER -