KP4 fungal toxin inhibits growth in Ustilago maydis by blocking calcium uptake

Matthew J Gage, Jeremy Bruenn, Marc Fischer, Dale Sanders, Thomas J. Smith

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

KP4 is a virally encoded fungal toxin secreted by the P4 killer strain of Ustilago maydis. From our previous structural studies, it seemed unlikely that KP4 acts by forming channels in the target cell membrane. Instead, KP4 was proposed to act by blocking fungal calcium channels, as KP4 was shown to inhibit voltage-gated calcium channels in rat neuronal cells, and its effects on fungal cells were abrogated by exogenously added calcium. Here, we extend these studies and demonstrate that KP4 acts in a reversible manner on the cell membrane and does not kill the cells, but rather inhibits cell division. This action is mimicked by EGTA and is abrogated specifically by low concentrations of calcium or non-specifically by high ionic strength buffers. We also demonstrate that KP4 affects 45Ca uptake in U. maydis. Finally, we show that cAMP and a cAMP analogue, N 6,2″-O-dibutyryladenosine 3′:5′-cyclic monophosphate, both abrogate KP4 effects. These results suggest that KP4 may inhibit cell growth and division by blocking calcium-regulated signal transduction pathways.

Original languageEnglish (US)
Pages (from-to)775-785
Number of pages11
JournalMolecular Microbiology
Volume41
Issue number4
DOIs
StatePublished - Dec 1 2001
Externally publishedYes

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Ustilago
Mycotoxins
Calcium Channels
Calcium
Cell Division
Growth
Cell Membrane
Egtazic Acid
Osmolar Concentration
Signal Transduction
Buffers

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

KP4 fungal toxin inhibits growth in Ustilago maydis by blocking calcium uptake. / Gage, Matthew J; Bruenn, Jeremy; Fischer, Marc; Sanders, Dale; Smith, Thomas J.

In: Molecular Microbiology, Vol. 41, No. 4, 01.12.2001, p. 775-785.

Research output: Contribution to journalArticle

Gage, Matthew J ; Bruenn, Jeremy ; Fischer, Marc ; Sanders, Dale ; Smith, Thomas J. / KP4 fungal toxin inhibits growth in Ustilago maydis by blocking calcium uptake. In: Molecular Microbiology. 2001 ; Vol. 41, No. 4. pp. 775-785.
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