KlebSeq, a diagnostic tool for surveillance, detection, and monitoring of Klebsiella pneumoniae

Jolene R. Bowers, Darrin Lemmer, Jason W. Sahl, Talima R Pearson, Elizabeth M. Driebe, Bette Wojack, Michael A. Saubolle, David M. Engelthaler, Paul S Keim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Health care-acquired infections (HAIs) kill tens of thousands of people each year and add significantly to health care costs. Multidrug-resistant and epidemic strains are a large proportion of HAI agents, and multidrug-resistant strains of Klebsiella pneumoniae, a leading HAI agent, have caused an urgent public health crisis. In the health care environment, patient colonization by K. pneumoniae precedes infection, and transmission via colonization leads to outbreaks. Periodic patient screening for K. pneumoniae colonization has the potential to curb the number of HAIs. In this report, we describe the design and validation of KlebSeq, a highly informative screening tool that detects Klebsiella species and identifies clinically important strains and characteristics by using highly multiplexed amplicon sequencing without a live-culturing step. We demonstrate the utility of this tool on several complex specimen types, including urine, wound swabs and tissue, and several types of respiratory and fecal specimens, showing K. pneumoniae species and clonal group identification and antimicrobial resistance and virulence profiling, including capsule typing. Use of this amplicon sequencing tool to screen patients for Klebsiella carriage could inform health care staff of the risk of infection and outbreak potential. KlebSeq also serves as a model for next-generation molecular tools for public health and health care, as expansion of this tool can be used for several other HAI agents or applications.

Original languageEnglish (US)
Pages (from-to)2582-2596
Number of pages15
JournalJournal of Clinical Microbiology
Volume54
Issue number10
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

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Klebsiella pneumoniae
Delivery of Health Care
Infection
Klebsiella
Disease Outbreaks
Public Health
Social Identification
Infectious Disease Transmission
Health Care Costs
Capsules
Virulence
Urine
Wounds and Injuries

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

KlebSeq, a diagnostic tool for surveillance, detection, and monitoring of Klebsiella pneumoniae. / Bowers, Jolene R.; Lemmer, Darrin; Sahl, Jason W.; Pearson, Talima R; Driebe, Elizabeth M.; Wojack, Bette; Saubolle, Michael A.; Engelthaler, David M.; Keim, Paul S.

In: Journal of Clinical Microbiology, Vol. 54, No. 10, 01.10.2016, p. 2582-2596.

Research output: Contribution to journalArticle

Bowers, JR, Lemmer, D, Sahl, JW, Pearson, TR, Driebe, EM, Wojack, B, Saubolle, MA, Engelthaler, DM & Keim, PS 2016, 'KlebSeq, a diagnostic tool for surveillance, detection, and monitoring of Klebsiella pneumoniae', Journal of Clinical Microbiology, vol. 54, no. 10, pp. 2582-2596. https://doi.org/10.1128/JCM.00927-16
Bowers, Jolene R. ; Lemmer, Darrin ; Sahl, Jason W. ; Pearson, Talima R ; Driebe, Elizabeth M. ; Wojack, Bette ; Saubolle, Michael A. ; Engelthaler, David M. ; Keim, Paul S. / KlebSeq, a diagnostic tool for surveillance, detection, and monitoring of Klebsiella pneumoniae. In: Journal of Clinical Microbiology. 2016 ; Vol. 54, No. 10. pp. 2582-2596.
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