Isoform-specific complementation of the yeast sac6 null mutation by human fimbrin

Alison Adams, Wenyan Shen, Ching Shwun Lin, John Leavitt, Paul Matsudaira

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The actin cytoskeleton is a fundamental component of eukaryotic cells, with both structural and motile roles. Actin and many of the actin-binding proteins found in different cell types are highly conserved, showing considerable similarity in both primary structure and biochemical properties. To make detailed comparisons between homologous proteins, it is necessary to know whether the various proteins are functionally, as well as structurally, conserved. Fimbrin is an example of a cytoskeletal component that, as shown by sequence determinations and biochemical characterizations, is conserved between organisms as diverse as Saccharomyces cerevisiae and humans. In this study, we examined whether the human homolog can substitute for the yeast protein in vivo. We report here that two isoforms of human fimbrin, also referred to as T- and L-plastin, can both substitute in vivo for yeast fimbrin, also known as Sac6p, whereas a third isoform, I-fimbrin (or I- plastin), cannot. We demonstrate that the human T- and L-fimbrins, in addition to complementing the temperature-sensitive growth defect of the sac6 null mutant, restore both normal cytoskeletal organization and cell shape to the mutant cells. In addition, we show that human T- and L-fimbrins can complement a sporulation defect caused by the sac6 null mutation. These findings indicate that there is a high degree of functional conservation in the cytoskeleton, even between organisms as diverse as S. cerevisiae and humans.

Original languageEnglish (US)
Pages (from-to)69-75
Number of pages7
JournalMolecular and Cellular Biology
Volume15
Issue number1
StatePublished - Jan 1995
Externally publishedYes

Fingerprint

Protein Isoforms
Yeasts
Mutation
Saccharomyces cerevisiae
plastin
Microfilament Proteins
Fungal Proteins
Cell Shape
Eukaryotic Cells
Cytoskeleton
Actin Cytoskeleton
Sequence Analysis
Actins
Proteins
Temperature
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Adams, A., Shen, W., Lin, C. S., Leavitt, J., & Matsudaira, P. (1995). Isoform-specific complementation of the yeast sac6 null mutation by human fimbrin. Molecular and Cellular Biology, 15(1), 69-75.

Isoform-specific complementation of the yeast sac6 null mutation by human fimbrin. / Adams, Alison; Shen, Wenyan; Lin, Ching Shwun; Leavitt, John; Matsudaira, Paul.

In: Molecular and Cellular Biology, Vol. 15, No. 1, 01.1995, p. 69-75.

Research output: Contribution to journalArticle

Adams, A, Shen, W, Lin, CS, Leavitt, J & Matsudaira, P 1995, 'Isoform-specific complementation of the yeast sac6 null mutation by human fimbrin', Molecular and Cellular Biology, vol. 15, no. 1, pp. 69-75.
Adams, Alison ; Shen, Wenyan ; Lin, Ching Shwun ; Leavitt, John ; Matsudaira, Paul. / Isoform-specific complementation of the yeast sac6 null mutation by human fimbrin. In: Molecular and Cellular Biology. 1995 ; Vol. 15, No. 1. pp. 69-75.
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