Intra- and extracellular dehydration has no effect on plasma levels of angiotensin II in an amphibian

Loretta P. Mayer, Catherine R Propper

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Previous studies have demonstrated that both dehydration (intra and extracellular) and treatment with angiotensin II (A-II) induce changes in thirst-related behavior in the spadefoot toad, Scaphiopus couchii. One of the steps in determining a causal relationship between a hormone and a behavior is to determine that there is association between an animal's performance of the behavior and changes in endogenous hormonal concentrations. The hypothesis tested that plasma levels of the peptide hormone A-II would change as a result of dehydration known to induce water absorption response (WR) behavior in the spadefoot toad. Plasma samples were taken from toads dehydrated intracellularly by injection of hypertonic solutions of NaCl or sucrose at levels known to induce WR behavior. As an osmotic control, a group of animals was injected with urea, which has been demonstrated to not induce WR behavior. In order to determine the effects of extracellular dehydration on plasma, A-II levels in toads dehydrated by plasma volume depletion via cardiac puncture were compared to sham-punctured controls. None of the treatments in any experiment resulted in significant differences in plasma levels of angiotensin II among groups sampled at the time when WR behavior occurs. These results do not support the hypothesis that dehydration-induced thirst is stimulated by changes in plasma A-II concentrations at the onset of WR behavior. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)343-349
Number of pages7
JournalJournal of Experimental Zoology
Volume286
Issue number4
DOIs
StatePublished - Mar 1 2000

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dehydration (animal physiology)
angiotensin II
amphibians
water uptake
toads
thirst
peptide hormones
animal performance
urea
hormones
sucrose
injection

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Intra- and extracellular dehydration has no effect on plasma levels of angiotensin II in an amphibian. / Mayer, Loretta P.; Propper, Catherine R.

In: Journal of Experimental Zoology, Vol. 286, No. 4, 01.03.2000, p. 343-349.

Research output: Contribution to journalArticle

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