Interactions Between the Yeast Ogataea pini and Filamentous Fungi Associated with the Western Pine Beetle

Thomas S. Davis, Richard Hofstetter, Jeffrey T Foster, Nathaniel E. Foote, Paul S Keim

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30 Citations (Scopus)

Abstract

Ecologically important microbes other than filamentous fungi can be housed within the fungal-transport structures (mycangia) of Dendroctonus bark beetles. The yeast Ogataea pini (Saccharomycetales: Saccharomycetaceae) was isolated from the mycangia of western pine beetle (Dendroctonus brevicomis) populations in northern Arizona (USA) with a frequency of 56%. We performed a series of in vitro assays to test whether volatile organic compounds produced by O. pini affected radial growth rates of mutualistic and antagonistic species of filamentous fungi that are commonly found in association with the beetle including Entomocorticium sp. B, Ophiostoma minus, Beauvaria bassiana, and an Aspergillus sp. We determined the compounds O. pini produced when grown on 2% malt extract agar using a gas chromatography/mass spectrometry (GC/MS) analysis of headspace volatiles. Volatiles produced by O. pini on artificial media significantly enhanced the growth of the mutualistic Entomocorticium sp. B, and inhibited growth of the entomopathogenic fungus B. bassiana. GC/MS revealed that O. pini produced ethanol, carbon disulfide (CS2), and Δ-3-carene in headspace. The results of these studies implicate O. pini as an important component in D. brevicomis community ecology, and we introduce multiple hypotheses for future tests of the effects of yeasts in the symbiont assemblages associated with Dendroctonus bark beetles.

Original languageEnglish (US)
Pages (from-to)626-634
Number of pages9
JournalMicrobial Ecology
Volume61
Issue number3
DOIs
StatePublished - Apr 2011

Fingerprint

Dendroctonus brevicomis
Dendroctonus
yeast
beetle
bark beetles
fungus
yeasts
headspace analysis
fungi
Saccharomycetales
Saccharomycetaceae
carbon disulfide
bark
Ophiostoma
carene
gas chromatography
malt extract
community ecology
transport structure
mass spectrometry

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

Cite this

Interactions Between the Yeast Ogataea pini and Filamentous Fungi Associated with the Western Pine Beetle. / Davis, Thomas S.; Hofstetter, Richard; Foster, Jeffrey T; Foote, Nathaniel E.; Keim, Paul S.

In: Microbial Ecology, Vol. 61, No. 3, 04.2011, p. 626-634.

Research output: Contribution to journalArticle

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