Incongruence between multi-locus sequence analysis (MLSA) and whole-genome-based phylogenies: Pseudomonas syringae pathovar pisi as a cautionary tale

David A. Baltrus, Kevin Dougherty, Stephen M Beckstrom-Sternberg, James S. Beckstrom-Sternberg, Jeffrey T Foster

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Summary: Previous phylogenies, built using a subset of genomic loci, split Pseudomonas syringae pv. pisi into two well-supported clades and implied convergence in host range for these lineages. The analysis of phenotypic and genotypic data within the context of this phylogenetic relationship implied further convergence at the level of virulence gene loss and acquisition. We generate draft genome assemblies for two additional P.syringae strains, isolated from diseased pea plants, and demonstrate incongruence between phylogenies created from a subset of the data compared with the whole genomes. Our whole-genome analysis demonstrates that strains classified as pv. pisi actually form a coherent monophyletic clade, so that apparent convergence is actually the product of shared ancestry. We use this example to urge caution when making evolutionary inferences across closely related strains of P.syringae. Molecular Plant Pathology

Original languageEnglish (US)
Pages (from-to)461-465
Number of pages5
JournalMolecular Plant Pathology
Volume15
Issue number5
DOIs
StatePublished - 2014

Fingerprint

Pseudomonas syringae
Phylogeny
Sequence Analysis
phylogeny
genome
Genome
Pseudomonas syringae pv. pisi
Plant Pathology
genome assembly
Plant Diseases
Molecular Pathology
Host Specificity
plant pathology
host range
Peas
pathology
virulence
ancestry
Virulence
peas

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science
  • Agronomy and Crop Science
  • Soil Science
  • Medicine(all)

Cite this

Incongruence between multi-locus sequence analysis (MLSA) and whole-genome-based phylogenies : Pseudomonas syringae pathovar pisi as a cautionary tale. / Baltrus, David A.; Dougherty, Kevin; Beckstrom-Sternberg, Stephen M; Beckstrom-Sternberg, James S.; Foster, Jeffrey T.

In: Molecular Plant Pathology, Vol. 15, No. 5, 2014, p. 461-465.

Research output: Contribution to journalArticle

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