Implantation of a three-dimensional fibroblast matrix improves left ventricular function and blood flow after acute myocardial infarction

Hoang M. Thai, Elizabeth Juneman, Jordan Lancaster, Tracy Hagerty, Rose Do, Lisa Castellano, Robert S Kellar, Stuart Williams, Gulshan Sethi, Monika Schmelz, Mohamed Gaballa, Steven Goldman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This study was designed to determine if a viable biodegradable three-dimensional fibroblast construct (3DFC) patch implanted on the left ventricle after myocardial infarction (MI) improves left ventricular (LV) function and blood flow. We ligated the left coronary artery of adult male Sprague-Dawley rats and implanted the 3DFC at the time of the infarct. Three weeks after MI, the 3DFC improved LV systolic function by increasing (p < 0.05) ejection fraction (37 ± 3% to 62 ± 5%), increasing regional systolic displacement of the infarcted wall (0.04 ± 0.02 to 0.11 ± 0.03 cm), and shifting the passive LV diastolic pressure volume relationship toward the pressure axis. The 3FDC improved LV remodeling by decreasing (p < 0.05) LV end-systolic and end-diastolic diameters with no change in LV systolic pressure. The 3DFC did not change LV end-diastolic pressure (LV EDP; 25 ± 2 vs. 23 ± 2 mmHg) but the addition of captopril (2mg/L drinking water) lowered (p < 0.05) LV EDP to 12.9 ± 2.5 mmHg and shifted the pressure-volume relationship toward the pressure axis and decreased (p < 0.05) the LV operating end-diastolic volume from 0.49 ± 0.02 to 0.34 ± 0.03 ml. The 3DFC increased myocardial blood flow to the infarcted anterior wall after MI over threefold (p < 0.05). This biodegradable 3DFC patch improves LV function and myocardial blood flow 3 weeks after MI. This is a potentially new approach to cell-based therapy for heart failure after MI.

Original languageEnglish (US)
Pages (from-to)283-295
Number of pages13
JournalCell Transplantation
Volume18
Issue number3
DOIs
StatePublished - 2009

Fingerprint

Fibroblasts
Left Ventricular Function
Blood
Myocardial Infarction
Ventricular Pressure
Blood Pressure
Pressure
Anterior Wall Myocardial Infarction
Ventricular Remodeling
Captopril
Cell- and Tissue-Based Therapy
Drinking Water
Stroke Volume
Heart Ventricles
Sprague Dawley Rats
Coronary Vessels
Heart Failure
Potable water
Rats

Keywords

  • Acute myocardial infarct
  • Angiogenesis
  • Bioabsorbable scaffold
  • Extracellular matrix
  • Fibroblasts
  • Growth factors

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Cite this

Implantation of a three-dimensional fibroblast matrix improves left ventricular function and blood flow after acute myocardial infarction. / Thai, Hoang M.; Juneman, Elizabeth; Lancaster, Jordan; Hagerty, Tracy; Do, Rose; Castellano, Lisa; Kellar, Robert S; Williams, Stuart; Sethi, Gulshan; Schmelz, Monika; Gaballa, Mohamed; Goldman, Steven.

In: Cell Transplantation, Vol. 18, No. 3, 2009, p. 283-295.

Research output: Contribution to journalArticle

Thai, HM, Juneman, E, Lancaster, J, Hagerty, T, Do, R, Castellano, L, Kellar, RS, Williams, S, Sethi, G, Schmelz, M, Gaballa, M & Goldman, S 2009, 'Implantation of a three-dimensional fibroblast matrix improves left ventricular function and blood flow after acute myocardial infarction', Cell Transplantation, vol. 18, no. 3, pp. 283-295. https://doi.org/10.3727/096368909788535004
Thai, Hoang M. ; Juneman, Elizabeth ; Lancaster, Jordan ; Hagerty, Tracy ; Do, Rose ; Castellano, Lisa ; Kellar, Robert S ; Williams, Stuart ; Sethi, Gulshan ; Schmelz, Monika ; Gaballa, Mohamed ; Goldman, Steven. / Implantation of a three-dimensional fibroblast matrix improves left ventricular function and blood flow after acute myocardial infarction. In: Cell Transplantation. 2009 ; Vol. 18, No. 3. pp. 283-295.
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