Impact of methylene chloride on microorganisms and phenanthrene mineralization in soil

Egbert Schwartz, Sinh V. Trinh, Kate M. Scow

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

This study investigated the effects of the quantity of methylene chloride, used as a carrier solvent for phenanthrene when added to soil, on phenanthrene mineralization kinetics, soil phospholipid fatty acid profiles (PLFA), and phenanthrene distribution. Methylene chloride dosages of 25 μL/g soil or more resulted in an enrichment of saturated PLFAs, suggesting soil microorganisms had adjusted their cell membranes in response to the solvent. A greater fraction of phenanthrene mineralized when spiked in 5 μL/g than in 25 μL/g methylene chloride suggesting that the methylene chloride became toxic to phenanthrene-degrading organisms in soil. Phenanthrene was more equally distributed among 0.1 g soil subsamples if spiked in 25 than 5 or 1 μL methylene chloride per gram soil. Thus the amount of methylene chloride used to spike phenanthrene in soil strongly impacted the mineralization kinetics, phenanthrene distribution, and microbial community in soil. Because a variety of spiking methods are used in biodegradation research, scientists should consider the quantity of solvents used when comparing results among different studies.

Original languageEnglish (US)
Pages (from-to)144-149
Number of pages6
JournalJournal of Environmental Quality
Volume31
Issue number1
StatePublished - 2002
Externally publishedYes

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ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry

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