Immunity in mice vaccinated with a molecular weight 60,000 glycoprotein secreted by adult Nematospiroides dubius

Fernando P Monroy, I. J. East, C. Dobson, J. H. Adams

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Immunity in mice vaccinated with a molecular weight 60,000 glycoprotein secreted by adult Nematospiroides dubius. International Journal for Parasitology 19: 71-76. A mol. wt 60,000 glycoprotein was purified from adult Nematospiroides dubius excretory-secretory products (ES) by polyacrylamide gel electrophoresis and electroelution and used to vaccinate BCF1 mice. This molecule is also released from the surface of the parasite and can be cleaved by pepsin. Mice immunized twice with ES 60,000 in Alum adjuvant harboured similar numbers of N. dubius as did control mice but voided 40% fewer parasite eggs in their faeces. The mol. wt 60,000 component in ES and from the surface of the parasite appears to influence how the parasite reproduces and may be important to its survival.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalInternational Journal for Parasitology
Volume19
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Nematospiroides dubius
Immunity
Glycoproteins
Parasites
Molecular Weight
Parasitology
Pepsin A
Feces
Eggs
Polyacrylamide Gel Electrophoresis

Keywords

  • excretory-secretory antigens
  • immunity
  • Nematospiroides dubius
  • vaccines

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Immunity in mice vaccinated with a molecular weight 60,000 glycoprotein secreted by adult Nematospiroides dubius. / Monroy, Fernando P; East, I. J.; Dobson, C.; Adams, J. H.

In: International Journal for Parasitology, Vol. 19, No. 1, 1989, p. 71-76.

Research output: Contribution to journalArticle

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