Identifying experimental surrogates for Bacillus anthracis spores: A review

David L. Greenberg, Joseph D. Busch, Paul S Keim, David M Wagner

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70 Scopus citations

Abstract

Bacillus anthracis, the causative agent of anthrax, is a proven biological weapon. In order to study this threat, a number of experimental surrogates have been used over the past 70 years. However, not all surrogates are appropriate for B. anthracis, especially when investigating transport, fate and survival. Although B. atrophaeus has been widely used as a B. anthracis surrogate, the two species do not always behave identically in transport and survival models. Therefore, we devised a scheme to identify a more appropriate surrogate for B. anthracis. Our selection criteria included risk of use (pathogenicity), phylogenetic relationship, morphology and comparative survivability when challenged with biocides. Although our knowledge of certain parameters remains incomplete, especially with regards to comparisons of spore longevity under natural conditions, we found that B. thuringiensis provided the best overall fit as a non-pathogenic surrogate for B. anthracis. Thus, we suggest focusing on this surrogate in future experiments of spore fate and transport modelling.

Original languageEnglish (US)
Article number4
JournalInvestigative Genetics
Volume1
Issue number1
DOIs
StatePublished - Sep 1 2010

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics
  • Molecular Biology

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