Identification, structure, and differential expression of members of a BURP domain containing protein family in soybean

Cheryl Granger, Virginia Coryell, Anupama Khanna, Paul S Keim, Lila Vodkin, Randy C. Shoemaker

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Expressed sequence tags (ESTs) exhibiting homology to a BURP domain containing gene family were identified from the Glycine max (L.) Merr. EST database. These ESTs were assembled into 16 contigs of variable sizes and lengths. Consistent with the structure of known BURP domain containing proteins, the translation products exhibit a modular structure consisting of a C-terminal BURP domain, an N-terminal signal sequence, and a variable internal region. The soybean family members exhibit 35-98% similarity in a ∼100-amino-acid C-terminal region, and a phylogenetic tree constructed using this region shows that some soybean family members group together in closely related pairs, triplets, and quartets, whereas others remain as singletons. The structure of these groups suggests that multiple gene duplication events occurred during the evolutionary history of this family. The depth and diversity of G. max EST libraries allowed tissue-specific expression patterns of the putative soybean BURPs to be examined. Consistent with known BURP proteins, the newly identified soybean BURPs have diverse expression patterns. Furthermore, putative paralogs can have both spatially and quantitatively distinct expression patterns. We discuss the functional and evolutionary implications of these findings, as well as the utility of EST-based analyses for identifying and characterizing gene families.

Original languageEnglish (US)
Pages (from-to)693-701
Number of pages9
JournalGenome
Volume45
Issue number4
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

expressed sequence tags
Soybeans
Expressed Sequence Tags
soybeans
proteins
Glycine max
Group Structure
gene duplication
signal peptide
Gene Duplication
translation (genetics)
Protein Biosynthesis
Protein Sorting Signals
genes
Genes
Protein Domains
History
amino acids
Databases
phylogeny

Keywords

  • BURP domain
  • Expressed sequence tag
  • Gene duplication
  • Glycine max

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

Identification, structure, and differential expression of members of a BURP domain containing protein family in soybean. / Granger, Cheryl; Coryell, Virginia; Khanna, Anupama; Keim, Paul S; Vodkin, Lila; Shoemaker, Randy C.

In: Genome, Vol. 45, No. 4, 2002, p. 693-701.

Research output: Contribution to journalArticle

Granger, Cheryl ; Coryell, Virginia ; Khanna, Anupama ; Keim, Paul S ; Vodkin, Lila ; Shoemaker, Randy C. / Identification, structure, and differential expression of members of a BURP domain containing protein family in soybean. In: Genome. 2002 ; Vol. 45, No. 4. pp. 693-701.
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