Identification of melioidosis outbreak by multilocus variable number tandem repeat analysis

Bart J. Currie, Asha Haslem, Talima R Pearson, Heidie Hornstra, Benjamin Leadem, Mark Mayo, Daniel Gal, Linda Ward, Daniel Godoy, Brian G. Spratt, Paul S Keim

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed- field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.

Original languageEnglish (US)
Pages (from-to)169-174
Number of pages6
JournalEmerging Infectious Diseases
Volume15
Issue number2
DOIs
StatePublished - Feb 2009

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Melioidosis
Minisatellite Repeats
Burkholderia pseudomallei
Multilocus Sequence Typing
Disease Outbreaks
Pulsed Field Gel Electrophoresis
Biological Warfare Agents
Drinking Water

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

Cite this

Identification of melioidosis outbreak by multilocus variable number tandem repeat analysis. / Currie, Bart J.; Haslem, Asha; Pearson, Talima R; Hornstra, Heidie; Leadem, Benjamin; Mayo, Mark; Gal, Daniel; Ward, Linda; Godoy, Daniel; Spratt, Brian G.; Keim, Paul S.

In: Emerging Infectious Diseases, Vol. 15, No. 2, 02.2009, p. 169-174.

Research output: Contribution to journalArticle

Currie, BJ, Haslem, A, Pearson, TR, Hornstra, H, Leadem, B, Mayo, M, Gal, D, Ward, L, Godoy, D, Spratt, BG & Keim, PS 2009, 'Identification of melioidosis outbreak by multilocus variable number tandem repeat analysis', Emerging Infectious Diseases, vol. 15, no. 2, pp. 169-174. https://doi.org/10.3201/eid1502.081036
Currie, Bart J. ; Haslem, Asha ; Pearson, Talima R ; Hornstra, Heidie ; Leadem, Benjamin ; Mayo, Mark ; Gal, Daniel ; Ward, Linda ; Godoy, Daniel ; Spratt, Brian G. ; Keim, Paul S. / Identification of melioidosis outbreak by multilocus variable number tandem repeat analysis. In: Emerging Infectious Diseases. 2009 ; Vol. 15, No. 2. pp. 169-174.
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