Hybridization in Populus alters the species composition and interactions of root-colonizing fungi

Consequences for host plant performance1

Catherine A Gehring, Baoming Ji, Sarah Fong, Thomas G Whitham

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Interactions among plants and soil microbes can significantly influence plant communities, yet we understand little about how hybridization of plant species might alter these interactions. In addition, few studies have explored the effects of different components of soil microbial communities on plant performance. We tested for feedbacks between soil microbes within a Populus hybridizing system using approaches that allowed us to isolate the effects of arbuscular mycorrhizal fungi (AMF) and root endophytes. We found significant differences among the arbuscular mycorrhizal (AM) fungal spore communities cultured from Populus angustifolia James, Populus fremontii S. Watson, and their F1 hybrids. Populus angustifolia cuttings grew 40% larger when inoculated with AM fungal spores from F1 hybrids than with spores from P. fremontii, while growth with spores from P. angustifolia was intermediate. However, parental and hybrid inocula promoted growth equally when soil inoculum was used. Roots inoculated with AM fungal spores alone were colonized principally by AMF, while those inoculated with soil were colonized mostly by dark septate endophytes. These results indicate that genetic variation among hybridizing plant species can influence both microbial communities and their interactions with host plants, but these effects depend upon the type of microbe. Furthermore, our results suggest that interactions among fungi during root colonization may alter the composition and function of the plant microbiome.

Original languageEnglish (US)
Pages (from-to)287-293
Number of pages7
JournalBotany
Volume92
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Populus
Populus angustifolia
host plant
spore
hybridization
host plants
fungus
fungal spores
species diversity
fungi
Populus fremontii
endophyte
endophytes
soil microorganisms
soil
mycorrhizal fungi
microbial communities
microbial community
inoculum
spores

Keywords

  • Arbuscular mycorrhizal fungi
  • Dark septate endophyte
  • Interspecific hybrid
  • Plant-soil feedback
  • Populus spp

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Ecology

Cite this

@article{aac8f75121d0496bbdc326447c57cf8a,
title = "Hybridization in Populus alters the species composition and interactions of root-colonizing fungi: Consequences for host plant performance1",
abstract = "Interactions among plants and soil microbes can significantly influence plant communities, yet we understand little about how hybridization of plant species might alter these interactions. In addition, few studies have explored the effects of different components of soil microbial communities on plant performance. We tested for feedbacks between soil microbes within a Populus hybridizing system using approaches that allowed us to isolate the effects of arbuscular mycorrhizal fungi (AMF) and root endophytes. We found significant differences among the arbuscular mycorrhizal (AM) fungal spore communities cultured from Populus angustifolia James, Populus fremontii S. Watson, and their F1 hybrids. Populus angustifolia cuttings grew 40{\%} larger when inoculated with AM fungal spores from F1 hybrids than with spores from P. fremontii, while growth with spores from P. angustifolia was intermediate. However, parental and hybrid inocula promoted growth equally when soil inoculum was used. Roots inoculated with AM fungal spores alone were colonized principally by AMF, while those inoculated with soil were colonized mostly by dark septate endophytes. These results indicate that genetic variation among hybridizing plant species can influence both microbial communities and their interactions with host plants, but these effects depend upon the type of microbe. Furthermore, our results suggest that interactions among fungi during root colonization may alter the composition and function of the plant microbiome.",
keywords = "Arbuscular mycorrhizal fungi, Dark septate endophyte, Interspecific hybrid, Plant-soil feedback, Populus spp",
author = "Gehring, {Catherine A} and Baoming Ji and Sarah Fong and Whitham, {Thomas G}",
year = "2014",
doi = "10.1139/cjb-2013-0174",
language = "English (US)",
volume = "92",
pages = "287--293",
journal = "Botany",
issn = "1916-2790",
publisher = "National Research Council of Canada",
number = "4",

}

TY - JOUR

T1 - Hybridization in Populus alters the species composition and interactions of root-colonizing fungi

T2 - Consequences for host plant performance1

AU - Gehring, Catherine A

AU - Ji, Baoming

AU - Fong, Sarah

AU - Whitham, Thomas G

PY - 2014

Y1 - 2014

N2 - Interactions among plants and soil microbes can significantly influence plant communities, yet we understand little about how hybridization of plant species might alter these interactions. In addition, few studies have explored the effects of different components of soil microbial communities on plant performance. We tested for feedbacks between soil microbes within a Populus hybridizing system using approaches that allowed us to isolate the effects of arbuscular mycorrhizal fungi (AMF) and root endophytes. We found significant differences among the arbuscular mycorrhizal (AM) fungal spore communities cultured from Populus angustifolia James, Populus fremontii S. Watson, and their F1 hybrids. Populus angustifolia cuttings grew 40% larger when inoculated with AM fungal spores from F1 hybrids than with spores from P. fremontii, while growth with spores from P. angustifolia was intermediate. However, parental and hybrid inocula promoted growth equally when soil inoculum was used. Roots inoculated with AM fungal spores alone were colonized principally by AMF, while those inoculated with soil were colonized mostly by dark septate endophytes. These results indicate that genetic variation among hybridizing plant species can influence both microbial communities and their interactions with host plants, but these effects depend upon the type of microbe. Furthermore, our results suggest that interactions among fungi during root colonization may alter the composition and function of the plant microbiome.

AB - Interactions among plants and soil microbes can significantly influence plant communities, yet we understand little about how hybridization of plant species might alter these interactions. In addition, few studies have explored the effects of different components of soil microbial communities on plant performance. We tested for feedbacks between soil microbes within a Populus hybridizing system using approaches that allowed us to isolate the effects of arbuscular mycorrhizal fungi (AMF) and root endophytes. We found significant differences among the arbuscular mycorrhizal (AM) fungal spore communities cultured from Populus angustifolia James, Populus fremontii S. Watson, and their F1 hybrids. Populus angustifolia cuttings grew 40% larger when inoculated with AM fungal spores from F1 hybrids than with spores from P. fremontii, while growth with spores from P. angustifolia was intermediate. However, parental and hybrid inocula promoted growth equally when soil inoculum was used. Roots inoculated with AM fungal spores alone were colonized principally by AMF, while those inoculated with soil were colonized mostly by dark septate endophytes. These results indicate that genetic variation among hybridizing plant species can influence both microbial communities and their interactions with host plants, but these effects depend upon the type of microbe. Furthermore, our results suggest that interactions among fungi during root colonization may alter the composition and function of the plant microbiome.

KW - Arbuscular mycorrhizal fungi

KW - Dark septate endophyte

KW - Interspecific hybrid

KW - Plant-soil feedback

KW - Populus spp

UR - http://www.scopus.com/inward/record.url?scp=84898748398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898748398&partnerID=8YFLogxK

U2 - 10.1139/cjb-2013-0174

DO - 10.1139/cjb-2013-0174

M3 - Article

VL - 92

SP - 287

EP - 293

JO - Botany

JF - Botany

SN - 1916-2790

IS - 4

ER -