High-resolution quantitative phase-contrast microscopy by digital holography

Christopher J Mann, Lingfeng Yu, Chun Min Lo, Myung K. Kim

Research output: Contribution to journalArticle

502 Citations (Scopus)

Abstract

Techniques of digital holography are improved in order to obtain high-resolution, high-fidelity images of quantitative phase-contrast microscopy. In particular, the angular spectrum method of calculating holographic optical field is seen to have significant advantages including tight control of spurious noise components. Holographic phase images are obtained with 0.5 μm diffraction-limited lateral resolution and largely immune from the coherent noise common in other holographic techniques. The phase profile is accurate to about 30 nm of optical thickness. Images of SKOV-3 ovarian cancer cells display intracellular and intranuclear organelles with clarity and quantitative accuracy.

Original languageEnglish (US)
Pages (from-to)8693-8698
Number of pages6
JournalOptics Express
Volume13
Issue number22
DOIs
StatePublished - Oct 31 2005
Externally publishedYes

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phase contrast
holography
microscopy
high resolution
clarity
organelles
optical thickness
cancer
profiles
diffraction

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

High-resolution quantitative phase-contrast microscopy by digital holography. / Mann, Christopher J; Yu, Lingfeng; Lo, Chun Min; Kim, Myung K.

In: Optics Express, Vol. 13, No. 22, 31.10.2005, p. 8693-8698.

Research output: Contribution to journalArticle

Mann, Christopher J ; Yu, Lingfeng ; Lo, Chun Min ; Kim, Myung K. / High-resolution quantitative phase-contrast microscopy by digital holography. In: Optics Express. 2005 ; Vol. 13, No. 22. pp. 8693-8698.
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