High carbon use efficiency in soil microbial communities is related to balanced growth, not storage compound synthesis

Paul Dijkstra, Elena Salpas, Dawson Fairbanks, Erin B. Miller, Shannon B. Hagerty, Kees Jan van Groenigen, Bruce A Hungate, Jane C Marks, George W Koch, Egbert Schwartz

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The efficiency with which microbes use substrate (Carbon Use Efficiency or CUE) to make new microbial biomass is an important variable in soil and ecosystem C cycling models. It is generally assumed that CUE of microbial activity in soils is low, however measured values vary widely. It is hypothesized that high values of CUE observed in especially short-term incubations reflect the build-up of storage compounds in response to a sudden increase in substrate availability and are therefore not representative of CUE of microbial activity in unamended soil.To test this hypothesis, we measured the <sup>13</sup>CO<inf>2</inf> release from six position-specific <sup>13</sup>C-labeled glucose isotopomers in ponderosa pine and piñon-juniper soil. We compared this position-specific CO<inf>2</inf> production pattern with patterns expected for 1) balanced microbial growth (synthesis of all compounds needed to build new microbial cells) at a low, medium, or high CUE, and 2) synthesis of storage compounds (glycogen, tri-palmitoyl-glycerol, and polyhydroxybutyrate).Results of this study show that synthesis of storage compounds is not responsible for the observed high CUE. Instead, it is the position-specific CO<inf>2</inf> production expected for balanced growth and high CUE that best matches the observed CO<inf>2</inf> production pattern in these two soils. Comparison with published studies suggests that the amount of glucose added in this study is too low and the duration of the experiment too short to affect microbial metabolism. We conclude that the hypothesis of high CUE in undisturbed soil microbial communities remains viable and worthy of further testing.

Original languageEnglish (US)
Pages (from-to)35-43
Number of pages9
JournalSoil Biology and Biochemistry
Volume89
DOIs
StatePublished - Oct 1 2015

Fingerprint

microbial communities
microbial community
Soil
Carbon
synthesis
carbon
Growth
soil
microbial activity
Pinus ponderosa
glucose
Mesons
polyhydroxybutyrate
Juniperus
Glucose
substrate
Glycogen
Biomass
glycogen
microbial biomass

Keywords

  • Fluxomics
  • Glycogen
  • Metabolic model
  • Polyhydroxybutyrate
  • Position-specific labeling
  • Tri-palmitoyl-glycerol

ASJC Scopus subject areas

  • Soil Science
  • Microbiology

Cite this

High carbon use efficiency in soil microbial communities is related to balanced growth, not storage compound synthesis. / Dijkstra, Paul; Salpas, Elena; Fairbanks, Dawson; Miller, Erin B.; Hagerty, Shannon B.; van Groenigen, Kees Jan; Hungate, Bruce A; Marks, Jane C; Koch, George W; Schwartz, Egbert.

In: Soil Biology and Biochemistry, Vol. 89, 01.10.2015, p. 35-43.

Research output: Contribution to journalArticle

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