Genotyping of Coxiella burnetii from domestic ruminants and human in Hungary

Indication of various genotypes

Kinga M. Sulyok, Zsuzsa Kreizinger, Heidie M. Hornstra, Talima R Pearson, Alexandra Szigeti, Ádám Dán, Eszter Balla, Paul S Keim, Miklós Gyuranecz

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Information about the genotypic characteristic of Coxiella burnetii from Hungary is lacking. The aim of this study is to describe the genetic diversity of C. burnetii in Hungary and compare genotypes with those found elsewhere. A total of 12 samples: (cattle, n = 6, sheep, n = 5 and human, n = 1) collected from across Hungary were studied by a 10-loci multispacer sequence typing (MST) and 6-loci multiple-locus variable-number of tandem repeat analysis (MLVA). Phylogenetic relationships among MST genotypes show how these Hungarian samples are related to others collected around the world.Results: Three MST genotypes were identified: sequence type (ST) 20 has also been identified in ruminants from other European countries and the USA, ST28 was previously identified in Kazakhstan, and the proposed ST37 is novel. All MST genotypes yielded different MLVA genotypes and three different MLVA genotypes were identified within ST20 samples alone. Two novel MLVA types 0-9-5-5-6-2 (AG) and 0-8-4-5-6-2 (AF) (Ms23-Ms24-Ms27-Ms28-Ms33-Ms34) were defined in the ovine materials correlated with ST28 and ST37. Samples from different parts of the phylogenetic tree were associated with different hosts, suggesting host-specific adaptations.Conclusions: Even with the limited number of samples analysed, this study revealed high genetic diversity among C. burnetii in Hungary. Understanding the background genetic diversity will be essential in identifying and controlling outbreaks.

Original languageEnglish (US)
Article number107
JournalBMC Veterinary Research
Volume10
DOIs
StatePublished - May 7 2014

Fingerprint

Coxiella burnetii
Hungary
Ruminants
genotyping
ruminants
Minisatellite Repeats
Genotype
genotype
genetic variation
Sheep
sampling
Furylfuramide
Kazakhstan
sheep
loci
phylogeny
Disease Outbreaks
multiple-locus variable number tandem-repeat analysis
cattle

Keywords

  • Coxiella burnetii
  • Genotyping
  • Hungary
  • MLVA
  • MST
  • Q fever

ASJC Scopus subject areas

  • veterinary(all)
  • Medicine(all)

Cite this

Genotyping of Coxiella burnetii from domestic ruminants and human in Hungary : Indication of various genotypes. / Sulyok, Kinga M.; Kreizinger, Zsuzsa; Hornstra, Heidie M.; Pearson, Talima R; Szigeti, Alexandra; Dán, Ádám; Balla, Eszter; Keim, Paul S; Gyuranecz, Miklós.

In: BMC Veterinary Research, Vol. 10, 107, 07.05.2014.

Research output: Contribution to journalArticle

Sulyok, Kinga M. ; Kreizinger, Zsuzsa ; Hornstra, Heidie M. ; Pearson, Talima R ; Szigeti, Alexandra ; Dán, Ádám ; Balla, Eszter ; Keim, Paul S ; Gyuranecz, Miklós. / Genotyping of Coxiella burnetii from domestic ruminants and human in Hungary : Indication of various genotypes. In: BMC Veterinary Research. 2014 ; Vol. 10.
@article{9bad741a525149bc8020f0ab6f428cb7,
title = "Genotyping of Coxiella burnetii from domestic ruminants and human in Hungary: Indication of various genotypes",
abstract = "Background: Information about the genotypic characteristic of Coxiella burnetii from Hungary is lacking. The aim of this study is to describe the genetic diversity of C. burnetii in Hungary and compare genotypes with those found elsewhere. A total of 12 samples: (cattle, n = 6, sheep, n = 5 and human, n = 1) collected from across Hungary were studied by a 10-loci multispacer sequence typing (MST) and 6-loci multiple-locus variable-number of tandem repeat analysis (MLVA). Phylogenetic relationships among MST genotypes show how these Hungarian samples are related to others collected around the world.Results: Three MST genotypes were identified: sequence type (ST) 20 has also been identified in ruminants from other European countries and the USA, ST28 was previously identified in Kazakhstan, and the proposed ST37 is novel. All MST genotypes yielded different MLVA genotypes and three different MLVA genotypes were identified within ST20 samples alone. Two novel MLVA types 0-9-5-5-6-2 (AG) and 0-8-4-5-6-2 (AF) (Ms23-Ms24-Ms27-Ms28-Ms33-Ms34) were defined in the ovine materials correlated with ST28 and ST37. Samples from different parts of the phylogenetic tree were associated with different hosts, suggesting host-specific adaptations.Conclusions: Even with the limited number of samples analysed, this study revealed high genetic diversity among C. burnetii in Hungary. Understanding the background genetic diversity will be essential in identifying and controlling outbreaks.",
keywords = "Coxiella burnetii, Genotyping, Hungary, MLVA, MST, Q fever",
author = "Sulyok, {Kinga M.} and Zsuzsa Kreizinger and Hornstra, {Heidie M.} and Pearson, {Talima R} and Alexandra Szigeti and {\'A}d{\'a}m D{\'a}n and Eszter Balla and Keim, {Paul S} and Mikl{\'o}s Gyuranecz",
year = "2014",
month = "5",
day = "7",
doi = "10.1186/1746-6148-10-107",
language = "English (US)",
volume = "10",
journal = "BMC Veterinary Research",
issn = "1746-6148",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Genotyping of Coxiella burnetii from domestic ruminants and human in Hungary

T2 - Indication of various genotypes

AU - Sulyok, Kinga M.

AU - Kreizinger, Zsuzsa

AU - Hornstra, Heidie M.

AU - Pearson, Talima R

AU - Szigeti, Alexandra

AU - Dán, Ádám

AU - Balla, Eszter

AU - Keim, Paul S

AU - Gyuranecz, Miklós

PY - 2014/5/7

Y1 - 2014/5/7

N2 - Background: Information about the genotypic characteristic of Coxiella burnetii from Hungary is lacking. The aim of this study is to describe the genetic diversity of C. burnetii in Hungary and compare genotypes with those found elsewhere. A total of 12 samples: (cattle, n = 6, sheep, n = 5 and human, n = 1) collected from across Hungary were studied by a 10-loci multispacer sequence typing (MST) and 6-loci multiple-locus variable-number of tandem repeat analysis (MLVA). Phylogenetic relationships among MST genotypes show how these Hungarian samples are related to others collected around the world.Results: Three MST genotypes were identified: sequence type (ST) 20 has also been identified in ruminants from other European countries and the USA, ST28 was previously identified in Kazakhstan, and the proposed ST37 is novel. All MST genotypes yielded different MLVA genotypes and three different MLVA genotypes were identified within ST20 samples alone. Two novel MLVA types 0-9-5-5-6-2 (AG) and 0-8-4-5-6-2 (AF) (Ms23-Ms24-Ms27-Ms28-Ms33-Ms34) were defined in the ovine materials correlated with ST28 and ST37. Samples from different parts of the phylogenetic tree were associated with different hosts, suggesting host-specific adaptations.Conclusions: Even with the limited number of samples analysed, this study revealed high genetic diversity among C. burnetii in Hungary. Understanding the background genetic diversity will be essential in identifying and controlling outbreaks.

AB - Background: Information about the genotypic characteristic of Coxiella burnetii from Hungary is lacking. The aim of this study is to describe the genetic diversity of C. burnetii in Hungary and compare genotypes with those found elsewhere. A total of 12 samples: (cattle, n = 6, sheep, n = 5 and human, n = 1) collected from across Hungary were studied by a 10-loci multispacer sequence typing (MST) and 6-loci multiple-locus variable-number of tandem repeat analysis (MLVA). Phylogenetic relationships among MST genotypes show how these Hungarian samples are related to others collected around the world.Results: Three MST genotypes were identified: sequence type (ST) 20 has also been identified in ruminants from other European countries and the USA, ST28 was previously identified in Kazakhstan, and the proposed ST37 is novel. All MST genotypes yielded different MLVA genotypes and three different MLVA genotypes were identified within ST20 samples alone. Two novel MLVA types 0-9-5-5-6-2 (AG) and 0-8-4-5-6-2 (AF) (Ms23-Ms24-Ms27-Ms28-Ms33-Ms34) were defined in the ovine materials correlated with ST28 and ST37. Samples from different parts of the phylogenetic tree were associated with different hosts, suggesting host-specific adaptations.Conclusions: Even with the limited number of samples analysed, this study revealed high genetic diversity among C. burnetii in Hungary. Understanding the background genetic diversity will be essential in identifying and controlling outbreaks.

KW - Coxiella burnetii

KW - Genotyping

KW - Hungary

KW - MLVA

KW - MST

KW - Q fever

UR - http://www.scopus.com/inward/record.url?scp=84900008737&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900008737&partnerID=8YFLogxK

U2 - 10.1186/1746-6148-10-107

DO - 10.1186/1746-6148-10-107

M3 - Article

VL - 10

JO - BMC Veterinary Research

JF - BMC Veterinary Research

SN - 1746-6148

M1 - 107

ER -