Genotyping of Burkholderia mallei from an Outbreak of Glanders in Bahrain Suggests Multiple Introduction Events

Holger C. Scholz, Talima Pearson, Heidie Hornstra, Michaela Projahn, Rahime Terzioglu, Renate Wernery, Enrico Georgi, Julia M. Riehm, David M. Wagner, Paul S. Keim, Marina Joseph, Bobby Johnson, Joerg Kinne, Shanti Jose, Crystal M. Hepp, Angela Witte, Ulrich Wernery

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Glanders, caused by the gram-negative bacterium Burkholderia mallei, is a highly infectious zoonotic disease of solipeds causing severe disease in animals and men. Although eradicated from many Western countries, it recently emerged in Asia, the Middle-East, Africa, and South America. Due to its rareness, little is known about outbreak dynamics of the disease and its epidemiology.

We investigated a recent outbreak of glanders in Bahrain by applying high resolution genotyping (multiple locus variable number of tandem repeats, MLVA) and comparative whole genome sequencing to B. mallei isolated from infected horses and a camel. These results were compared to samples obtained from an outbreak in the United Arab Emirates in 2004, and further placed into a broader phylogeographic context based on previously published B. mallei data. The samples from the outbreak in Bahrain separated into two distinct clusters, suggesting a complex epidemiological background and evidence for the involvement of multiple B. mallei strains. Additionally, the samples from Bahrain were more closely related to B. mallei isolated from horses in the United Arab Emirates in 2004 than other B. mallei which is suggestive of repeated importation to the region from similar geographic sources.

High-resolution genotyping and comparative whole genome analysis revealed the same phylogenetic patterns among our samples. The close relationship of the Dubai/UAE B. mallei populations to each other may be indicative of a similar geographic origin that has yet to be identified for the infecting strains. The recent emergence of glanders in combination with worldwide horse trading might pose a new risk for human infections.

Original languageEnglish (US)
JournalPLoS Neglected Tropical Diseases
Volume8
Issue number9
DOIs
StatePublished - Sep 1 2014

Fingerprint

Burkholderia mallei
Glanders
Bahrain
Disease Outbreaks
United Arab Emirates
Horses
Genome
Camelus
Eastern Africa
Minisatellite Repeats
Animal Diseases
Middle East
South America
Zoonoses
Gram-Negative Bacteria
Epidemiology

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Genotyping of Burkholderia mallei from an Outbreak of Glanders in Bahrain Suggests Multiple Introduction Events. / Scholz, Holger C.; Pearson, Talima; Hornstra, Heidie; Projahn, Michaela; Terzioglu, Rahime; Wernery, Renate; Georgi, Enrico; Riehm, Julia M.; Wagner, David M.; Keim, Paul S.; Joseph, Marina; Johnson, Bobby; Kinne, Joerg; Jose, Shanti; Hepp, Crystal M.; Witte, Angela; Wernery, Ulrich.

In: PLoS Neglected Tropical Diseases, Vol. 8, No. 9, 01.09.2014.

Research output: Contribution to journalArticle

Scholz, HC, Pearson, T, Hornstra, H, Projahn, M, Terzioglu, R, Wernery, R, Georgi, E, Riehm, JM, Wagner, DM, Keim, PS, Joseph, M, Johnson, B, Kinne, J, Jose, S, Hepp, CM, Witte, A & Wernery, U 2014, 'Genotyping of Burkholderia mallei from an Outbreak of Glanders in Bahrain Suggests Multiple Introduction Events', PLoS Neglected Tropical Diseases, vol. 8, no. 9. https://doi.org/10.1371/journal.pntd.0003195
Scholz, Holger C. ; Pearson, Talima ; Hornstra, Heidie ; Projahn, Michaela ; Terzioglu, Rahime ; Wernery, Renate ; Georgi, Enrico ; Riehm, Julia M. ; Wagner, David M. ; Keim, Paul S. ; Joseph, Marina ; Johnson, Bobby ; Kinne, Joerg ; Jose, Shanti ; Hepp, Crystal M. ; Witte, Angela ; Wernery, Ulrich. / Genotyping of Burkholderia mallei from an Outbreak of Glanders in Bahrain Suggests Multiple Introduction Events. In: PLoS Neglected Tropical Diseases. 2014 ; Vol. 8, No. 9.
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