Genomic sequences of six botulinum neurotoxin-producing strains representing three clostridial species illustrate the mobility and diversity of botulinum neurotoxin genes

Theresa J. Smith, Karen K. Hill, Gary Xie, Brian T. Foley, Charles H D Williamson, Jeffrey T Foster, Shannon L. Johnson, Olga Chertkov, Hazuki Teshima, Henry S. Gibbons, Lauren A. Johnsky, Mark A. Karavis, Leonard A. Smith

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The whole genomes for six botulinum neurotoxin-producing clostridial strains were sequenced to provide references for under-represented toxin types, bivalent strains or unusual toxin complexes associated with a bont gene. The strains include three Clostridium botulinum Group I strains (CDC 297, CDC 1436, and Prevot 594), a Group II C. botulinum strain (Eklund 202F), a Group IV Clostridium argentinense strain (CDC 2741), and a Group V Clostridium baratii strain (Sullivan). Comparisons of the Group I genomic sequences revealed close relationships and conservation of toxin gene locations with previously published Group I C. botulinum genomes. The bont/F6 gene of strain Eklund 202F was determined to be a chimeric toxin gene composed of bont/F1 and bont/F2. The serotype G strain CDC 2741 remained unfinished in 20 contigs with the bont/G located within a 1.15. Mb contig, indicating a possible chromosomal location for this toxin gene. Within the genome of C. baratii Sullivan strain, direct repeats of IS1182 insertion sequence (IS) elements were identified flanking the bont/F7 toxin complex that may be the mechanism of bont insertion into C. baratii. Highlights of the six strains are described and release of their genomic sequences will allow further study of unusual neurotoxin-producing clostridial strains.

Original languageEnglish (US)
Pages (from-to)102-113
Number of pages12
JournalInfection, Genetics and Evolution
Volume30
DOIs
StatePublished - Mar 1 2015

Fingerprint

botulinum toxin
Neurotoxins
toxin
Centers for Disease Control and Prevention (U.S.)
Clostridium botulinum
genomics
gene
Tetanus Toxin
Clostridium
Genes
Clostridium baratii
Genome
toxins
genes
genome
Immunotoxins
DNA Transposable Elements
Nucleic Acid Repetitive Sequences
Clostridium argentinense
neurotoxins

Keywords

  • Botulinum neurotoxin
  • Clostridium argentinense
  • Clostridium baratii
  • Clostridium botulinum

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Genomic sequences of six botulinum neurotoxin-producing strains representing three clostridial species illustrate the mobility and diversity of botulinum neurotoxin genes. / Smith, Theresa J.; Hill, Karen K.; Xie, Gary; Foley, Brian T.; Williamson, Charles H D; Foster, Jeffrey T; Johnson, Shannon L.; Chertkov, Olga; Teshima, Hazuki; Gibbons, Henry S.; Johnsky, Lauren A.; Karavis, Mark A.; Smith, Leonard A.

In: Infection, Genetics and Evolution, Vol. 30, 01.03.2015, p. 102-113.

Research output: Contribution to journalArticle

Smith, TJ, Hill, KK, Xie, G, Foley, BT, Williamson, CHD, Foster, JT, Johnson, SL, Chertkov, O, Teshima, H, Gibbons, HS, Johnsky, LA, Karavis, MA & Smith, LA 2015, 'Genomic sequences of six botulinum neurotoxin-producing strains representing three clostridial species illustrate the mobility and diversity of botulinum neurotoxin genes', Infection, Genetics and Evolution, vol. 30, pp. 102-113. https://doi.org/10.1016/j.meegid.2014.12.002
Smith, Theresa J. ; Hill, Karen K. ; Xie, Gary ; Foley, Brian T. ; Williamson, Charles H D ; Foster, Jeffrey T ; Johnson, Shannon L. ; Chertkov, Olga ; Teshima, Hazuki ; Gibbons, Henry S. ; Johnsky, Lauren A. ; Karavis, Mark A. ; Smith, Leonard A. / Genomic sequences of six botulinum neurotoxin-producing strains representing three clostridial species illustrate the mobility and diversity of botulinum neurotoxin genes. In: Infection, Genetics and Evolution. 2015 ; Vol. 30. pp. 102-113.
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abstract = "The whole genomes for six botulinum neurotoxin-producing clostridial strains were sequenced to provide references for under-represented toxin types, bivalent strains or unusual toxin complexes associated with a bont gene. The strains include three Clostridium botulinum Group I strains (CDC 297, CDC 1436, and Prevot 594), a Group II C. botulinum strain (Eklund 202F), a Group IV Clostridium argentinense strain (CDC 2741), and a Group V Clostridium baratii strain (Sullivan). Comparisons of the Group I genomic sequences revealed close relationships and conservation of toxin gene locations with previously published Group I C. botulinum genomes. The bont/F6 gene of strain Eklund 202F was determined to be a chimeric toxin gene composed of bont/F1 and bont/F2. The serotype G strain CDC 2741 remained unfinished in 20 contigs with the bont/G located within a 1.15. Mb contig, indicating a possible chromosomal location for this toxin gene. Within the genome of C. baratii Sullivan strain, direct repeats of IS1182 insertion sequence (IS) elements were identified flanking the bont/F7 toxin complex that may be the mechanism of bont insertion into C. baratii. Highlights of the six strains are described and release of their genomic sequences will allow further study of unusual neurotoxin-producing clostridial strains.",
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