Genomic organization and evolution of the soybean SB92 satellite sequence

Mahmood Vahedian, Liang Shi, Tong Zhu, Ronald Okimoto, Kathleen Danna, Paul S Keim

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Abstract

Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 105 copies. This repeated sequence is tandemly arranged and is found in four or five major genomic locations. FISH analysis of metaphase chromosomes suggests that two of these locations are centromeric. We have determined the sequence of two cloned repeats and performed genomic sequencing to obtain a consensus sequence. The consensus repeat size was 92 bp and exhibited an average of 10% nucleotide substitution relative to the two cloned repeats. This high level of sequence diversity suggests an ancient origin but is inconsistent with the limited phylogenetic distribution of SB92, which is found an high copy number only in the annual soybeans. It therefore seems likely that this sequence is undergoing very rapid evolution.

Original languageEnglish (US)
Pages (from-to)857-862
Number of pages6
JournalPlant Molecular Biology
Volume29
Issue number4
DOIs
StatePublished - Nov 1995

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Keywords

  • centromere
  • FISH
  • satellite DNA
  • SB92
  • soybean
  • tandemly repeated DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

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