Genomic analysis of the emergence and rapid global dissemination of the clonal group 258 Klebsiella pneumoniae pandemic

Jolene R. Bowers, Brandon Kitchel, Elizabeth M. Driebe, Duncan R. MacCannell, Chandler Roe, Darrin Lemmer, Tom De Man, J. Kamile Rasheed, David M. Engelthaler, Paul S Keim, Brandi M. Limbago

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Multidrug-resistant Klebsiella pneumoniae producing the KPC carbapenemase have rapidly spread throughout the world, causing severe healthcare-associated infections with limited antimicrobial treatment options. Dissemination of KPC-producing K. pneumoniae is largely attributed to expansion of a single dominant strain, ST258. In this study, we explore phylogenetic relationships and evolution within ST258 and its clonal group, CG258, using whole genome sequence analysis of 167 isolates from 20 countries collected over 17 years. Our results show a common ST258 ancestor emerged from its diverse parental clonal group around 1995 and likely acquired blaKPC prior to dissemination. Over the past two decades, ST258 has remained highly clonal despite diversity in accessory elements and divergence in the capsule polysaccharide synthesis locus. Apart from the large recombination event that gave rise to ST258, few mutations set it apart from its clonal group. However, one mutation occurs in a global transcription regulator. Characterization of outer membrane protein sequences revealed a profile in ST258 that includes a truncated OmpK35 and modified OmpK37. Our work illuminates potential genomic contributors to the pathogenic success of ST258, helps us better understand the global dissemination of this strain, and identifies genetic markers unique to ST258.

Original languageEnglish (US)
Article number0133727
JournalPLoS One
Volume10
Issue number7
DOIs
StatePublished - Jul 21 2015
Externally publishedYes

Fingerprint

Klebsiella pneumoniae
Pandemics
pandemic
mutation
genomics
Mutation
beta-lactamase
outer membrane proteins
Accessories
Transcription
Cross Infection
Genetic Markers
health services
Genetic Recombination
Capsules
Polysaccharides
Sequence Analysis
Membrane Proteins
ancestry
polysaccharides

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Bowers, J. R., Kitchel, B., Driebe, E. M., MacCannell, D. R., Roe, C., Lemmer, D., ... Limbago, B. M. (2015). Genomic analysis of the emergence and rapid global dissemination of the clonal group 258 Klebsiella pneumoniae pandemic. PLoS One, 10(7), [0133727]. https://doi.org/10.1371/journal.pone.0133727

Genomic analysis of the emergence and rapid global dissemination of the clonal group 258 Klebsiella pneumoniae pandemic. / Bowers, Jolene R.; Kitchel, Brandon; Driebe, Elizabeth M.; MacCannell, Duncan R.; Roe, Chandler; Lemmer, Darrin; De Man, Tom; Rasheed, J. Kamile; Engelthaler, David M.; Keim, Paul S; Limbago, Brandi M.

In: PLoS One, Vol. 10, No. 7, 0133727, 21.07.2015.

Research output: Contribution to journalArticle

Bowers, JR, Kitchel, B, Driebe, EM, MacCannell, DR, Roe, C, Lemmer, D, De Man, T, Rasheed, JK, Engelthaler, DM, Keim, PS & Limbago, BM 2015, 'Genomic analysis of the emergence and rapid global dissemination of the clonal group 258 Klebsiella pneumoniae pandemic', PLoS One, vol. 10, no. 7, 0133727. https://doi.org/10.1371/journal.pone.0133727
Bowers, Jolene R. ; Kitchel, Brandon ; Driebe, Elizabeth M. ; MacCannell, Duncan R. ; Roe, Chandler ; Lemmer, Darrin ; De Man, Tom ; Rasheed, J. Kamile ; Engelthaler, David M. ; Keim, Paul S ; Limbago, Brandi M. / Genomic analysis of the emergence and rapid global dissemination of the clonal group 258 Klebsiella pneumoniae pandemic. In: PLoS One. 2015 ; Vol. 10, No. 7.
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