Genetic analysis of the fimbrin-actin binding interaction in Saccharomyces cerevisiae

S. M. Brower, J. E. Honts, Alison Adams

Research output: Contribution to journalArticle

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Abstract

Yeast fimbrin is encoded by the SAC6 gene, mutations of which suppress temperature-sensitive mutations in the actin gene (ACT1). To examine the mechanism of suppression, we have sequenced 17 sac6 suppressor alleles, and found that they change nine different residues, all of which cluster in three regions of one of the two actin-binding domains of Sac6p. Two of these clusters occur in highly conserved regions (ABS1 and ABS3) that have been strongly implicated in the binding of related proteins to actin. The third cluster changes residues not previously implicated in the interaction with actin. As changes in any of nine different residues can suppress several different act1 alleles, it is likely that the suppressors restore the overall affinity, rather than specific lost interactions, between Sac6p and actin. Using mutagenesis, we have identified two mutations of the second actin- binding domain that can also suppress the act1 mutations of interest. This result suggests the two actin-binding domains of Sac6p interact with the same region of the actin molecule. However, differences in strength of suppression of temperature sensitivity and sporulation indicate that the two actin- binding domains are distinct, and explain why second-domain mutations were not identified previously.

Original languageEnglish (US)
Pages (from-to)91-101
Number of pages11
JournalGenetics
Volume140
Issue number1
StatePublished - 1995
Externally publishedYes

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Saccharomyces cerevisiae
Actins
Mutation
Alleles
plastin
Temperature
Mutagenesis
Genes
Carrier Proteins
Yeasts

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Genetic analysis of the fimbrin-actin binding interaction in Saccharomyces cerevisiae. / Brower, S. M.; Honts, J. E.; Adams, Alison.

In: Genetics, Vol. 140, No. 1, 1995, p. 91-101.

Research output: Contribution to journalArticle

Brower, S. M. ; Honts, J. E. ; Adams, Alison. / Genetic analysis of the fimbrin-actin binding interaction in Saccharomyces cerevisiae. In: Genetics. 1995 ; Vol. 140, No. 1. pp. 91-101.
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